Mineralization of collagen may occur on fibril surfaces: Evidence from conventional and high-voltage electron microscopy and three-dimensional imaging
Mineralization of collagen may occur on fibril surfaces: Evidence from conventional and high-voltage electron microscopy and three-dimensional imaging
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DOI：
10.1006/jsbi.1996.0066
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发表时间：
1996-07-01
影响因子：
3
通讯作者：
McEwen, BF
中科院分区：
文献类型：
--
作者：
Landis, WJ;Hodgens, KJ;McEwen, BF
用常规和高压电子显微镜、计算机断层扫描和三维图像重建等方法，在超微结构水平上研究了家火鸡正常钙化的腿部肌腱中胶原蛋白和矿物晶体之间的相互作用。对用水或无水技术处理的标本进行适当的切片，并拍摄早期肌腱矿化的区域。基于单独的显微照片、立体图像对和断层三维图像重建，可能首次发现与胶原纤维表面结合的板状晶体。在胶原蛋白空洞和重叠区内也可以观察到紧密平行的矿物质排列。肌腱中这些空间上不同位置的矿物质沉积为钙化提供了可能的途径，作为脊椎动物骨骼和牙齿形成的基础事件，胶原是钙化的中介。(C)1996年学术出版社。
The interaction between collagen and mineral crystals in the normally calcifying leg tendons from the domestic turkey, Meleagris gallopavo has been investigated at an ultrastructural level with conventional and high-voltage electron microscopy, computed tomography, and three-dimensional image reconstruction methods. Specimens treated by either aqueous or anhydrous techniques and resin-embedded were appropriately sectioned and regions of early tendon mineralization were photographed. On the basis of individual photomicrographs, stereoscopic pairs of images, and tomographic three-dimensional image reconstructions, platelet-shaped crystals may be demonstrated for the first time in association with the surface of collagen fibrils. Mineral is also observed in closely parallel arrays within collagen hole and overlap zones. The mineral deposition at these spatially distinct locations in the tendon provides insight into possible means by which calcification is mediated by collagen as a fundamental event in skeletal and dental formation among vertebrates. (C) 1996 Academic Press, Inc.