Challenges to Implementing Environmental-DNA Monitoring in Namibia
Challenges to Implementing Environmental-DNA Monitoring in Namibia
复制标题
DOI：
10.3389/fenvs.2021.773991
复制
发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Iain Perry;Ifan B. Jâms;R. Casas‐Mulet;J. Hamutoko;A. Marchbank;Selma Lendelvo;E. Naomab;B. Mapani-B.
中科院分区：
文献类型：
--
作者：
Iain Perry;Ifan B. Jâms;R. Casas‐Mulet;J. Hamutoko;A. Marchbank;Selma Lendelvo;E. Naomab;B. Mapani-B.
通过识别环境中的DNA片段，EDNA方法为以具有成本效益的方式监测生物多样性提供了一种很有前途的工具。这对于那些传统的形态监测稀少的国家来说尤其重要。实现EDNA潜力的第一步是开发适合当地情况的方法。在这里，我们在纳米比亚的一系列半干旱生态系统中测试现场和实验室EDNA协议(水样和沉积物样本)。我们成功地收集了EDNA关于多种营养水平(包括藻类、无脊椎动物和细菌)的广泛生物的EDNA数据，但确定了EDNA方法在该地区实施的两个关键挑战：1)高浊度需要量身定制的采样技术；2)EDNA方法识别分类群目前受到缺乏参考数据的限制。我们希望这项工作将指导在纳米比亚及其邻国的干旱生态系统中部署EDNA生物监测系统。
By identifying fragments of DNA in the environment, eDNA approaches present a promising tool for monitoring biodiversity in a cost-effective way. This is particularly pertinent for countries where traditional morphological monitoring has been sparse. The first step to realising the potential of eDNA is to develop methodologies that are adapted to local conditions. Here, we test field and laboratory eDNA protocols (aqueous and sediment samples) in a range of semi-arid ecosystems in Namibia. We successfully gathered eDNA data on a broad suite of organisms at multiple trophic levels (including algae, invertebrates and bacteria) but identified two key challenges to the implementation of eDNA methods in the region: 1) high turbidity requires a tailored sampling technique and 2) identification of taxa by eDNA methods is currently constrained by a lack of reference data. We hope this work will guide the deployment of eDNA biomonitoring in the arid ecosystems of Namibia and neighbouring countries.