Adipogenic Differentiation of Human Adipose-Derived Stem Cells on 3D Silk Scaffolds
Adipogenic Differentiation of Human Adipose-Derived Stem Cells on 3D Silk Scaffolds
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DOI：
10.1007/978-1-61737-960-4_23
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发表时间：
2011-01-01
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影响因子：
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通讯作者：
Kaplan, David L.
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文献类型：
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作者：
Choi, Jennifer H.;Bellas, Evangelia;Kaplan, David L.
由于各种病理和创伤导致的软组织缺损，目前的治疗方式包括自体移植以及使用市售的填充剂。然而，这些治疗方法存在一些局限性，比如供体部位的并发症以及随时间推移出现的体积损失。因此，需要改进的治疗方案。组织工程技术通过开发具有适当结构和功能、能够再生脂肪组织的生物活性组织构建体，为这些问题提供了新的解决方案。人类脂肪干细胞（hASCs）的获取和特性研究方面的最新进展，已经引发了众多关于软组织重建的研究。在这一章中，我们将讨论我们实验室使用人类脂肪干细胞和丝素支架进行脂肪组织工程的方法。将诸如丝蛋白这类天然存在且临床可接受的材料用于组织工程应用，在生物相容性以及机械和生物学特性方面具有优势。
Current treatment modalities for soft tissue defects due to various pathologies and trauma include autologous grafting and the use of commercially available fillers. However, these treatment methods are associated with a number of limitations, such as donor site morbidity and volume loss over time. As such, improved therapeutic options are needed. Tissue engineering techniques offer novel solutions to these problems through development of bioactive tissue constructs that can regenerate adipose tissue with an appropriate structure and function. The recent advances in the derivation and characterization of hASCs have led to numerous studies of soft tissue reconstruction. In this chapter, we discuss methods in which our laboratory has used hASCs and silk scaffolds for adipose tissue engineering. The use of naturally occurring and clinically acceptable materials such as silk protein for tissue-engineering applications poses advantages with respect to biocompatibility and mechanical and biological properties.