Agonist antibody activates death receptor 6 downstream signaling involving TRADD recruitment
Agonist antibody activates death receptor 6 downstream signaling involving TRADD recruitment
复制标题
激动剂抗体激活涉及 TRADD 募集的死亡受体 6 下游信号传导
DOI：
10.1016/j.febslet.2013.12.010
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发表时间：
2014-01-31
期刊：
影响因子：
3.5
通讯作者：
Zhang, Liguo
中科院分区：
文献类型：
--
作者：
Hu, Rui;Du, Qiumei;Zhang, Liguo
死亡受体6（DR6）是TNFR超家族中含有死亡结构域的受体。迄今为止，DR6的配体仍然没有明确定义。在这里，我们开发了一个功能激动剂单克隆抗体（DQM 3）的DR6，它结合到第一个富含半胱氨酸的结构域。重要的是，DR6信号可以被DQM3明显激活，这依赖于其细胞内死亡结构域。此外，我们证明DR6和TRADD之间的关联在DQM3刺激后增强，TRADD参与DR6诱导的信号激活。总之，我们的研究结果提供了一个新的洞察到一个新的机制，其中DR6诱导下游信号在响应激动剂antibody.Structured summary of protein interactions：DR6 physically interacts with TRADD by antibait coimmunoprecipitation（View interaction）（C）2013 Federation of European Biochemical Societies. Elsevier B.V.出版，保留所有权利。
Death receptor 6 (DR6) is a member of the death domain-containing receptors that belong to the TNFR superfamily. To date, the ligand for DR6 is still not clearly defined. Here, we developed a functional agonist monoclonal antibody (DQM3) against DR6, which bound to the first cysteine-rich domain. Importantly, DR6 signaling could be clearly activated by DQM3, which was dependent on its intracellular death domain. In addition, we demonstrated that the association between DR6 and TRADD was enhanced upon DQM3 stimulation and TRADD was involved in DR6-induced signaling activation. Taken together, our findings provide new insight into a novel mechanism by which DR6 induces downstream signaling in response to an agonist antibody.Structured summary of protein interactions:DR6 physically interacts with TRADD by anti bait coimmunoprecipitation (View interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.