Cloning of duck PRL cDNA and genomic DNA
Cloning of duck PRL cDNA and genomic DNA
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DOI：
10.1016/j.ygcen.2004.11.017
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发表时间：
2005-03-01
影响因子：
2.7
通讯作者：
Shimada, K
中科院分区：
文献类型：
--
作者：
Kansaku, N;Ohkubo, T;Shimada, K
克隆了家鸭催乳素（PRL）基因的cDNA和基因组DNA及其侧翼序列。鸭PRL分别为92.0、91.7。与鸡、火鸡和鹌鹑的PRL在cDNA水平上的同源性分别为91.4%。预测的氨基酸序列与鸡（93.4%），火鸡（91.3%）和鹌鹑（91.3%）PRL的可比区域具有总体相似性。成熟鸭PRL在第6位含有N-连接糖基化的共有序列，这在鸡或火鸡中都不存在。因此，鸭PRL很可能是不同的其他鸟类物种的post-diabetonally修改。根据cDNA序列，表征了该基因的基因组结构。鸭PRL基因由5个外显子和4个内含子组成。此外，鸭PRL启动子近端区域的序列分析显示，高度的相似性，鸡和火鸡PRL启动子。这些结果表明，机制，调节PRL基因的表达，可能是广泛保守的鸟类物种。(C)2004爱思唯尔公司All rights reserved.
Complementary DNA (cDNA) and genomic DNA, including flanking regions of the prolactin (PRL) gene of domesticated duck, were cloned and sequenced. Duck PRL was found to have 92.0, 91.7. and 91.4% sequence identity at the cDNA level to PRL of chicken, turkey, and quail, respectively. The predicted amino acid sequence had an overall similarity with a comparable region of chicken (93.4%), turkey (91.3%), and quail (91.3%) PRL. Mature duck PRL contains the consensus sequence for N-linked glycoslylation at position 6 which is not present in either chickens or turkeys. Thus, duck PRL is likely to be post-translationally modified differently from other avian species. Based on the cDNA sequence, the genomic structure of the gene was characterized. The duck PRL gene consists of 5 exons and 4 introns. Moreover, sequence analysis of the proximal region of duck PRL promoter revealed a high degree of similarity to that of chicken and turkey PRL promoter. These results suggest that the mechanisms, which regulate expression of the PRL gene, may be widely conserved in avian species. (C) 2004 Elsevier Inc. All rights reserved.