Upcycling of poly(ethylene terephthalate) to produce high-value bio-products
Upcycling of poly(ethylene terephthalate) to produce high-value bio-products
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DOI：
10.1016/j.celrep.2022.111908
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发表时间：
2022-12-29
期刊：
影响因子：
8.8
通讯作者：
Moon, Tae Seok
中科院分区：
文献类型：
--
作者：
Diao, Jinjin;Hu, Yifeng;Moon, Tae Seok
全球每年生产的聚对苯二甲酸乙酯（PET）超过7000万吨。PET废弃物的积累已成为全球关注的污染问题，促使人们迫切需要开发消费后PET的技术。化学催化和酶解方法的发展为PET通过生物转化转化为相应的单体开辟了新的机会。本研究鉴定出可直接利用PET水解物作为唯一碳源的约氏红球菌（RPET）菌株。我们还研究了RPET将PET升级为增值化学品的潜力，使用番茄红素作为概念验证产品。通过合理的代谢工程，使番茄红素产量比野生型提高500倍以上。此外，我们证明了通过将该菌株与PET碱性水解级联，从PET中生产约1,300 mg/L的番茄红素。这项工作强调了生物转化作为实现PET升级回收的一种手段的巨大潜力。
More than 70 million tons of poly(ethylene terephthalate) (PET) are manufactured worldwide every year. The accumulation of PET waste has become a global pollution concern, motivating the urgent development of technologies to valorize post-consumer PET. The development of chemocatalytic and enzymatic ap-proaches for depolymerizing PET to its corresponding monomers opens up new opportunities for PET upcy-cling through biological transformation. Here, we identify Rhodococcus jostii strain PET (RPET) that can directly use PET hydrolysate as a sole carbon source. We also investigate the potential of RPET to upcycle PET into value-added chemicals, using lycopene as a proof-of-concept product. Through rational metabolic engineering, we improve lycopene production by more than 500-fold over that of the wild type. In addition, we demonstrate the production of approximately 1,300 mg/L lycopene from PET by cascading this strain with PET alkaline hydrolysis. This work highlights the great potential of biological conversion as a means of achieving PET upcycling.