Distribution of low molecular weight platelet aggregation inhibitors from snake venoms
Distribution of low molecular weight platelet aggregation inhibitors from snake venoms
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DOI：
10.1016/j.toxicon.2006.09.027
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发表时间：
2007-03-01
期刊：
影响因子：
2.8
通讯作者：
Takahashi, Hidenobu
中科院分区：
文献类型：
--
作者：
Oyama, Etsuko;Takahashi, Hidenobu
用Aggregeter PAM 8C(Mebanix)比浊仪测定了5 7种蛇毒粗品经微孔UFP 1 LGC超滤后的血小板聚集抑制物含量。蛇毒对ADP诱导的兔血小板聚集有明显的抑制作用，分别为三种蛇毒、一种蛇毒和两种蛇毒。特别是，六种毒液对血小板聚集有较强的抑制作用。此外，采用高效液相色谱和FAB/MS联用分析方法，进一步鉴定了该毒液中的腺苷成分。(C)2006爱思唯尔有限公司。保留所有权利。
An assay of platelet aggregation inhibitors measured by the turbidimeter using Aggregometer PAM 8C (Mebanix) was performed after each crude snake venom (57 species) was subjected to ultrafiltration using MILLIPORE UFP 1 LGC. The snake venoms of Viperidae (three species), Elapidae (I I species), and Hydrophiidae (two species) inhibited ADP-induced rabbit platelet aggregation. In particular, six venoms of Bitis gabonica, Pseudocerastes persicus, Dendroaspis angusticeps, D. polylepis, Ophiophagus hannah, and N. nigricollis craivshaivii strongly inhibited platelet aggregation. Furthermore, adenosine was identified from Bitis gabonica venom using HPLC and FAB/MS analysis. (c) 2006 Elsevier Ltd. All rights reserved.