Factors Affecting Blind Localization of a Glass Micropipette Using a High-Density Microelectrode Array
Factors Affecting Blind Localization of a Glass Micropipette Using a High-Density Microelectrode Array
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影响使用高密度微电极阵列的玻璃微量移液器盲定位的因素
DOI：
10.1109/icsens.2013.6688361
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
U.
中科院分区：
文献类型：
--
作者：
Obien;M. E. J.;Hierlemann;A.;& Frey;U.
高密度微电极阵列（HDMEAs）提供了以高时空分辨率测量脑切片和分离细胞培养物的细胞外电位的能力，这对神经科学具有吸引力。由于HDMEA能够以亚细胞分辨率记录单个神经元的活动，因此与细胞内记录技术（例如膜片钳）的组合将允许新的体外实验。这种组合技术需要精确定位的细胞和玻璃微量移液管（GM）相对于HDMEA。在这里，我们提出了一种方法来定位的三维（3D）位置的GM上的HDMEA，而不使用光学显微镜。对于（x，y）位置，实现的精度为（±2μm，±5μm），小于18μm的电极间距。对于z位置，GM尖端和HDMEA表面之间5-50μm距离的精度为±2μm。我们还观察到GM尖端和HDMEA电极的尺寸变化对盲定位性能的影响最小。该方法显示了在HDMEA顶部自动导航GM以在体外修补单个细胞的可行性。
High-density microelectrode arrays (HDMEAs) provide the capability to measure extracellular electric potential from brain slices and dissociated cell cultures at high spatiotemporal resolution, which is attractive for neuroscience. Since the HDMEA enables to record the activity of single neurons at sub-cellular resolution, a combination with intracellular recording techniques, such as patch clamp, will allow for new in vitro experiments. Such combination technique requires precise localization of both the cell of interest and the glass micropipette (GM) with respect to the HDMEA. Here, we present a methodology to locate the three-dimensional (3D) position of a GM on the HDMEA without the use of an optical microscope. For the (x, y) position, the achieved accuracy is (±2μm, ±5μm), which is less than the electrode pitch of 18μm. For the z-position, the obtained accuracy is ±2μm for distances of 5-50μm between the GM tip and the HDMEA surface. We also observed that variations in size of GM tips and HDMEA electrodes have minimal effects on the blind localization performance. This approach shows the feasibility of automated navigation of a GM atop the HDMEA to patch a single cell in vitro.