Triiodothyronine stimulation of in vitro growth and maturation of embryonic chick cartilage.
Triiodothyronine stimulation of in vitro growth and maturation of embryonic chick cartilage.
复制标题
三碘甲状腺原氨酸刺激胚胎鸡软骨的体外生长和成熟。
DOI：
10.1210/endo-111-2-462
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发表时间：
1982
期刊：
影响因子：
4.8
通讯作者：
H. E. Lebovitz
中科院分区：
文献类型：
--
作者：
Warner M. Burch;H. E. Lebovitz
我们在体外研究了 T3 对软骨生长和成熟的直接影响。来自 9 天鸡胚的骨盆软骨在无血清器官培养系统中培养，该系统支持软骨在 5 天的时间间隔内生长。与单独在培养基中孵育的软骨相比，在培养基中添加 T3 (15 nM) 在 3 天内增加了软骨湿重（约 100%）、干重（77%）、长度（35%）和总可溶性蛋白（67%）。在此期间，T3 的 DNA 含量仅略有增加 (2%)。孵育 5 天后，T3 类似地刺激相同的生长参数。此外，T3 增加了[14C]亮氨酸与蛋白质的结合（82%）和35SO4与蛋白多糖的结合（53%）。在孵育 3 天后，T3 (0.0015-15 nM) 观察到软骨湿重呈剂量依赖性增加。与 T3 一起孵育的软骨显示出成熟过程中的微观变化，出现大量肥大的软骨细胞，并且成熟的生化证据显示碱性磷酸酶活性增加（130％）。 T3 刺激碱性磷酸酶活性的剂量反应范围（0.015-0.15 nM）比刺激生长的剂量反应范围（0.0015-15 nM）要严格得多。这些研究表明，生理浓度的 T3 主要通过刺激软骨细胞肥大直接影响软骨生长和成熟。
We studied the direct effect of T3 on cartilage growth and maturation in vitro. Pelvic cartilages from 9-day-old chick embryos were incubated in a serum-free organ culture system which supported cartilage growth over a 5-day interval. The addition of T3 (15 nM) to the medium increased cartilage wet weight (approximately 100%), dry weight (77%), length (35%), and total soluble protein (67%) over 3 days compared to cartilage incubated in medium alone. The DNA content was only slightly increased (2%) by T3 over the interval. T3 stimulated the same parameters of growth similarly after 5 days of incubation. In addition, T3 increased the incorporation of [14C]leucine into protein (82%) and 35SO4 into proteoglycan (53%). A dose-dependent increase in cartilage wet weight was seen with T3 (0.0015-15 nM) over 3 days of incubation. Cartilage incubated with T3 demonstrated microscopic changes in maturation, with development of large numbers of hypertrophied chondrocytes, and biochemical evidence of maturation, with increased alkaline phosphatase activity (130%). The dose-response range for T3 stimulation of alkaline phosphatase activity (0.015-0.15 nM) was considerably more restricted than that for stimulation of growth (0.0015-15 nM). These studies demonstrate that T3 in physiological concentrations directly affects cartilage growth and maturation, primarily through stimulating chondrocyte hypertrophy.