Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences
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DOI：
10.1038/s41598-020-57885-z
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发表时间：
2020-01-24
影响因子：
4.6
通讯作者：
De Beule, Pieter A. A.
中科院分区：
文献类型：
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作者：
Gomez-Varela, Ana I.;Stamov, Dimitar R.;De Beule, Pieter A. A.
来自不同显微镜技术的相关数据有可能发现细胞生物学中信号事件的新方面。在这里，我们报告的第一次硬件设置能够实现空间相关的远场超分辨率荧光显微镜和原子力显微镜，一个壮举，直到现在才获得的空间分辨率限制的阿贝衍射极限的荧光显微镜设置的同时共定位成像。我们详细的系统集成和展示系统性能，使用亚分辨率荧光珠和应用到测试样品组成的人骨肉瘤上皮细胞，质膜转运蛋白1（MCT 1）与增强型绿色荧光蛋白（EGFP）在N-末端标记。
Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit. We detail system integration and demonstrate system performance using sub-resolution fluorescent beads and applied to a test sample consisting of human bone osteosarcoma epithelial cells, with plasma membrane transporter 1 (MCT1) tagged with an enhanced green fluorescent protein (EGFP) at the N-terminal.