PHOSPHORYLATION OF ALPHA-CRYSTALLIN-B IN ALEXANDERS DISEASE BRAIN
PHOSPHORYLATION OF ALPHA-CRYSTALLIN-B IN ALEXANDERS DISEASE BRAIN
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DOI：
10.1016/0014-5793(91)81359-g
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发表时间：
1991-12-02
期刊：
影响因子：
3.5
通讯作者：
SPECTOR, A
中科院分区：
文献类型：
--
作者：
MANN, E;MCDERMOTT, MJ;SPECTOR, A
The phosphorylation of alpha-crystallin B was studied in homogenates of autopsy samples of brain tissue from patients with Alexander's disease, a condition characterized by over-expression of this protein. After incubation in the presence of [gamma-P-32]ATP and cAMP the homogenates were analyzed by two-dimensional electrophoresis, (isoelectric focusing followed by SDS-PAGE). Three major polypeptides having the same molecular weight as bovine lens alpha-crystallin B and pIs 7.1, 6.9 and 6.7 were detected in the Coomassie blue stained gels. These three polypeptides were recognized by an alpha-crystallin B-specific antiserum in Western blots. The polypeptides with pIs 7.1 and 6.7 co-migrated in isoelectric focusing gels with bovine lens alpha-B and its phosphorylated form alpha-Bp, respectively. Radioautography of the two-dimensional gels demonstrated the presence of P-32 in the most acidic polypeptide. The results demonstrate the occurrence of alpha-B phosphorylation in Alexander's disease brain tissue.
