Fabrication of Three-dimensional Deformable Microfilter for Capturing Target Cells
Fabrication of Three-dimensional Deformable Microfilter for Capturing Target Cells
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用于捕获目标细胞的三维可变形微过滤器的制造
DOI：
10.1109/mhs.2017.8305259
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yoshitaka Nakanishi
中科院分区：
文献类型：
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作者：
Kenshiro Nakatake;Yuta Nakashima;Ryo Iwamoto;Ayase Tashima;Yusuke Kitamura;Keiichiro Yasuda;Masaaki Iwatsuki;Hideo Baba;Toshihiro Ihara;Yoshitaka Nakanishi
本文提出了一种三维变形的微过滤器修饰的核酸适体捕获的目标细胞。从液体中分离和捕获靶细胞对于通过液体活检进行健康诊断和医学诊断非常重要。所提出的微过滤器允许任意尺寸的分离，因为微过滤器的开口间隙通过流体力三维地变换。微过滤器的变形能够通过调节流体力来改变。此外，所提出的微滤器允许通过核酸适体的特异性吸附能力特异性捕获靶细胞。在该实验中，癌细胞被用作靶细胞。使用对上皮细胞粘附分子具有特异性吸附能力的核酸适体来捕获癌细胞。实验结果表明，核酸适体修饰的微滤膜能有效地捕获靶细胞。
This paper presents a three-dimensional deformable microfilter modified with a nucleic-acid aptamer for capturing of target cells. Separating and capturing target cells from the fluid is very important for health diagnosis and medical diagnosis by liquid biopsy. The presented microfilter permits the separation of arbitrary size because the opening gap of the microfilter is three-dimensionally transformed by fluidic force. The deformation of the microfilter is able to be changed by adjusting the fluidic force. Also, the presented microfilter permits the specific capturing of target cells by specifically adsorbing ability of a nucleic-acid aptamer. In this experiment, the cancer cell was used as a target cell. The nucleic-acid aptamer that has a specific adsorbing ability to epithelial cell adhesion molecules was used for capturing the cancer cell. The results of experiment, the target cells were effectively captured by nucleic-acid aptamer modified microfilter.