Transcription Termination Factor Rho: A Ring-Shaped RNA Helicase from Bacteria
Transcription Termination Factor Rho: A Ring-Shaped RNA Helicase from Bacteria
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DOI：
10.1039/9781849732215-00243
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Boudvillain, Marc
中科院分区：
文献类型：
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作者：
Rabhi, Makhlouf;Rahmouni, A. Rachid;Boudvillain, Marc
许多依赖NTP的蛋白质机器形成类似甜甜圈的寡聚体结构。1-3这种环形机器包括DNA移位酶，例如病毒DNA包装马达或染色体分离酶以及参与重组和复制的许多DNA解旋酶。1，6-8相反，已知只有少数作用于RNA的NTPases形成寡聚环。9-12这些蛋白质包括参与（+）链RNA病毒复制和/或包装的病毒解旋酶、猴病毒40（一种双链DNA病毒）的大T抗原、囊病毒科的P4包装马达蛋白（我们未发表的观察结果和参考文献16;另见第9章）。来自大肠杆菌的转录终止因子Rho也是一种环状的六聚体酶，在体外显示ATP依赖性RNA和RNA-DNA解旋酶活性。17，18自1969年发现以来，19 Rho已成为经典的转录因子范例。Rho的主要公认的生物学功能是破坏
Many NTP-dependent protein machines form oligomeric structures resembling doughnouts. 1-3 Such ring-shaped machines include DNA translocases such as viral DNA packaging motors or chromosomal segregation enzymes as well as numerous DNA helicases involved in recombination and replication. 1, 6–8 In contrast, only a few NTPases working on RNA are known to form oligomeric rings. 9-12 These include viral helicases involved in the replication and/or packaging of (+)-strand RNA viruses, 13, 14 the large T antigen from simian virus 40 (a double-stranded DNA virus), 15 and the P4 packaging motor pro-teins from Cystoviridae phages (our unpublished observations and ref. 16; see also Chapter 9). The transcription termination factor Rho from Escherichia coli, the subject of this review, is also a ring-shaped, hexameric enzyme that displays ATP-dependent RNA and RNA-DNA helicase activities in vitro. 17, 18 Since its discovery in 1969, 19 Rho has become a classical transcription factor paradigm. The main, accepted biological function of Rho is the disruption of