Two CLE genes are induced by phosphate in roots of Lotus japonicus
Two CLE genes are induced by phosphate in roots of Lotus japonicus
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DOI：
10.1007/s10265-010-0342-5
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Kawaguchi, Masayoshi
中科院分区：
文献类型：
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作者：
Funayama-Noguchi, Sachiko;Noguchi, Ko;Kawaguchi, Masayoshi
CLE（CLAVATA 3/ESR-related）家族的基因编码肽配体，所述肽配体调节植物响应于外部刺激（例如根瘤菌感染和硝酸盐施用）以及各种内部刺激的发育。为了研究LjCLE基因是否参与植物对无机磷的反应，我们分析了39个LjCLE基因在水培百脉根（Miyakojima 'MG-20'）中的磷反应。两个LjCLE基因LjCLE 19和20在L.通过向溶液培养溶液中添加Pi来控制。当外源磷水平增加时，LjCLE 19和20的表达量先于磷含量增加而增加。另一方面，当外部Pi水平降低时，植物中的Pi含量首先降低，然后LjCLE 19和20的表达水平降低。根据我们的研究结果，我们讨论了LjCLE 19和20与植物组织磷水平的关系。这是第一份显示磷酸盐诱导特定CLE基因的报告。
Genes of CLE (CLAVATA3/ESR-related) family encode peptide ligands that regulate plant development in response to external stimuli such as rhizobial infection and the nitrate application as well as various internal stimuli. To investigate whether LjCLE gene(s) may involve in plant response to inorganic phosphate (Pi), we analyzed Pi responses of 39 LjCLE genes in hydroponically grown Lotus japonicus plants (ecotype Miyakojima 'MG-20'). Two LjCLE genes, LjCLE19 and 20, were up-regulated specifically and greatly in roots of L. japonicus by Pi addition to the hydroponic solution. When the external Pi level increased, expressions of LjCLE19 and 20 increased before the increase in the Pi content in plants. On the other hand, when the external Pi level decreased, the Pi content in plants decreased first, then expression levels of LjCLE19 and 20 decreased. Based on our results, we discuss the relationship between LjCLE19 and 20 and the tissue Pi levels in plants. This is the first report showing induction of specific CLE genes by phosphate.