Dynamic methylation pattern of cyp19a1a core promoter during zebrafish ovarian folliculogenesis
Dynamic methylation pattern of cyp19a1a core promoter during zebrafish ovarian folliculogenesis
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DOI：
10.1007/s10695-015-0187-x
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发表时间：
2016-06-01
影响因子：
2.9
通讯作者：
Chen, Shi Xi
中科院分区：
文献类型：
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作者：
Bai, Jin;Gong, Weida;Chen, Shi Xi
在脊椎动物中，cyp19a1a基因编码的芳香化酶可以催化雄激素转化为雌激素。因此，cyp19a1a基因表达的调控机制是生殖内分泌学的重要研究领域。本研究以斑马鱼为模型，研究cyp19a1a基因核心启动子在斑马鱼卵泡发育过程中的动态甲基化水平。结果表明，斑马鱼cyp19a1a核心启动子甲基化水平存在明显的波动。此外，当卵泡从PV发育到MV阶段时，甲基化水平与cyp19a1a转录本的表达水平呈负相关。此外，靠近转录起始位点的CpG二核苷酸可能对抑制RNA聚合酶II的转录功能提供了显著的阻断作用。综上所述，本研究的结果有力地表明，DNA甲基化是参与调控cyp19a1a基因表达在卵泡发育过程中的机制之一。这种甲基化机制改变了斑马鱼卵泡发育过程中的转录过程，也可能为其他脊椎动物卵巢发育阶段cyp19a1a的表达调控提供新的线索。
In vertebrates, the aromatase coded by the cyp19a1a gene can catalyze the conversion from androgens to estrogens. Thus, the regulatory mechanisms of cyp19a1a gene expression are a critical research field in reproductive endocrinology. In this study, we use zebrafish as a model to study the dynamic methylation levels of the cyp19a1a gene core promoter during zebrafish ovarian folliculogenesis. The results show that there is an apparent fluctuation of the methylation levels of zebrafish cyp19a1a core promoter. Moreover, the methylation levels are inversely correlated with the expression levels of cyp19a1a transcripts when the ovarian follicles develop from PV into the MV stage. Also, the CpG dinucleotides which are close to the transcriptional starting site may have provided a significant blocking effect on inhibiting the transcriptional function of RNA polymerase II. Taken together, the results from the present study strongly suggest that DNA methylation was one of mechanisms that are involved in the regulation of cyp19a1a gene expression during folliculogenesis. This methylation mechanism modifying transcriptional process accompanied with zebrafish ovarian folliculogenesis might also shed new light on the regulation of cyp19a1a expression during the ovarian developmental stage in other vertebrates.