The initial immune response during experimental cysticercosis is of the mixed Th1/Th2 type.
The initial immune response during experimental cysticercosis is of the mixed Th1/Th2 type.
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实验性囊尾蚴病期间的初始免疫反应是混合 Th1/Th2 类型。
DOI：
10.1007/s00436-002-0788-z
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发表时间：
2003
影响因子：
2
通讯作者：
Kuhn,RE
中科院分区：
文献类型：
--
作者：
Toenjes,SA;Kuhn,RE
实验性囊尾蚴病初始阶段发生的免疫学事件尚不清楚。本文介绍的研究检查了幼虫青皮绦虫 BALB/cJ 小鼠感染第一周期间腹膜渗出细胞 (PEC)、脾细胞和肠系膜淋巴结 (MLN) 细胞产生的细胞因子。增殖测定确定，可以测量抗原特异性反应的最早时间是感染后 5 天。伴刀豆球蛋白 A (ConA) 对宿主细胞的刺激在感染 24 小时时引发了 IL-4 的初始爆发，并且在感染后 7 天，ConA 刺激的 Th2 型细胞因子的产生占主导地位。因此，感染第一天的反应似乎促进了 Th2 型反应。用幼虫抗原刺激 MLN 细胞、脾细胞和 PEC 支持了先前关于混合 Th1/Th2 型细胞因子产生以及白细胞介素 (IL)-4、IL-10 和干扰素 (IFN)-γ 增加的报道。感染后 3、5 和 7 天，受感染小鼠 PEC 的离体 IFN-γ 产量增加，而在这些时间观察到离体 IL-10 产量减少。在寄生虫特异性和刀豆蛋白 A 诱导的增殖测定中，感染后 3 至 7 天期间，这种离体 IFN-γ 反应先于 PEC 产生的 IL-10 增加。因此，感染幼虫T。粗头虫会引起由 IFN-γ 介导的初始反应，随后很快就会增加 IL-10 的产生，并随后减少 IFN-γ 的产生量。
The immunological events that occur during the initial stages of experimental cysticercosis are not known. The studies presented here examined the cytokines produced by peritoneal exudate cells (PECs), splenocytes and mesenteric lymph node (MLN) cells during the first week of infection with larvalTaenia crassicepsin BALB/cJ mice. Proliferation assays determined that the earliest time when antigen-specific responses could be measured was 5 days post-infection. Concanavalin A (ConA) stimulation of host cells elicited an initial burst of IL-4 production at 24 h of infection and ConA-stimulated Th2-type cytokine production is predominant by 7 days post-infection. Thus, there are responses at day 1 of infection that seem to promote a Th2-type response. Stimulation of MLN cells, splenocytes and PECs with larval antigens supported previous reports of mixed Th1/Th2-type cytokine production with increases in interleukin (IL)-4, IL-10 and interferon (IFN)-γ. Ex vivo IFN-γ production by PECs from infected mice was increased at 3, 5 and 7 days post-infection, whereas at these times reduced ex vivo IL-10 production was observed. This ex vivo IFN-γ response preceded an increasing IL-10 production by PECs between 3 and 7 days post-infection in parasite-specific and ConA-induced proliferation assays. Thus, infection with larvalT. crassicepsresults in an initial response mediated by IFN-γ that is quickly followed by an increase in IL-10 production and subsequent reduction in the amount of IFN-γ being produced.