Fluorescent Carbon Dot-Curcumin Nanocomposites for Remarkable Antibacterial Activity with Synergistic Photodynamic and Photothermal Abilities
Fluorescent Carbon Dot-Curcumin Nanocomposites for Remarkable Antibacterial Activity with Synergistic Photodynamic and Photothermal Abilities
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DOI：
10.1021/acsabm.1c00377
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发表时间：
2021-08-16
影响因子：
4.7
通讯作者：
Su, Wei
中科院分区：
文献类型：
--
作者：
Yan, Hongjun;Zhang, Baoqu;Su, Wei
光敏剂（PS）介导的光动力疗法（PDT）作为传统抗生素治疗的替代疗法越来越受到人们的关注。然而，传统光敏剂的局限性严重阻碍了其实际应用，因此，如何提高传统光敏剂的抗菌性能已成为光医学领域的研究热点。本文构建了一种具有光动力和光热（PTT）协同抗菌作用的复合纳米ps体系，该体系由双波长照明触发。合成了荧光碳点（CDs）作为姜黄素（Cur）的载体，得到了CDs/Cur。在近红外和405 nm可见光双波长联合照射下，CDs/Cur可同时产生ROS并适度升温，对革兰氏阳性菌和革兰氏阴性菌均有协同抗菌作用。扫描电镜和荧光共聚焦成像结果显示，CDs/Cur与PDT和PTT的联合作用对细胞膜的损伤更为严重。此外，CDs/Cur具有较低的细胞毒性和可忽略的溶血活性，具有良好的生物相容性。因此，利用CDs作为光敏剂传递载体构建CDs/Cur，为提高光敏剂的抗菌效果和设计下一代光医学抗菌剂提供了一种策略。
Photosensitizer (PS)-mediated photodynamic therapy (PDT) has attracted more and more attention as an alternative to traditional antibiotic therapy. Nevertheless, the limitations of traditional photosensitizers seriously hinder their practical application, as a result, the methods to improve the antibacterial properties of traditional photosensitizers have become a hot topic in the field of photomedicine. Herein, a compound nano-PS system has been constructed with synergistic photodynamic and photothermal (PTT) antibacterial effects, triggered by a dual-wavelength illumination. Fluorescent carbon dots (CDs) were synthesized and employed as carriers for the delivery of curcumin (Cur) to obtain CDs/Cur. Upon combined near-infrared and 405 nm visible dual-wavelength irradiation, CDs/Cur could simultaneously generate ROS and a moderate temperature increase, triggering synergistic antibacterial effects against both Gram-positive and Gram-negative bacteria. The results of scanning electron microscopy and fluorescence confocal imaging showed that the combined effect of CDs/Cur with PDT and PTT caused more serious damage to the cell membrane. In addition, CDs/Cur exhibited low cytotoxicity and negligible hemolytic activity, showing great biocompatibility. Therefore, the construction of CDs/Cur by employing CDs as photosensitizer delivery carriers provides a strategy for the improvement of the antibacterial effect of the photosensitizer and the design of next-generation antibacterial agents in photomedicine.