Antigenic diversity and seroprevalences of Torque teno viruses in children and adults by ORF2-based immunoassays
Antigenic diversity and seroprevalences of Torque teno viruses in children and adults by ORF2-based immunoassays
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DOI：
10.1099/vir.0.046862-0
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发表时间：
2013-02-01
影响因子：
3.8
通讯作者：
Soderlund-Venermo, Maria
中科院分区：
文献类型：
--
作者：
Chen, Tingting;Vaisanen, Elina;Soderlund-Venermo, Maria
细环病毒（TTV）在人类中广泛传播，在健康个体中引起持续的病毒血症。许多TTV分离物具有高遗传变异性，已被鉴定为5个主要系统发育组的29个种。迄今为止，TTV序列的多样性、蛋白表达的挑战以及随后的血清学检测的缺乏阻碍了TTV血清阳性率的研究。此外，不同TTV的抗原关系及其在人类中的特异性血清阳性率仍然未知。对于属于四个基因组的不同物种的五个TTV株，我们开发了使用重组谷胱甘肽S-转移酶（GST）融合TTV ORF 2蛋白，谷胱甘肽GST捕获酶免疫测定法（EIA）检测针对构象表位的抗体。然后，我们分析了178名健康成人和108名儿童的血清样本;观察到IgG反应性，无论是对单一菌株或对多个菌株，这表明TTV物种的抗原性差异。在2-4岁儿童中，五种TTV的总体血清阳性率达到43%（42例中的18例），随后下降，在成人中再次达到42%（178例中的74例）。TTV 6种特异性IgG在儿童中占优势，而TTV 13在成人中占优势。在同一儿童的3年随访期间，发生了种特异性血清转化和血清逆转。这是第一个基于EIA的不同TTV的研究，为TTV感染的血清流行病学和诊断提供了新的方法。我们的数据表明，不同的TTV在人类中的抗病毒抗体谱，感染模式和流行病学可能有所不同。
Torque teno viruses (TTVs) circulate widely among humans, causing persistent viraemia in healthy individuals. Numerous TTV isolates with high genetic variability have been identified and segregated into 29 species of five major phylogenetic groups. To date, the diversity of TTV sequences, challenges in protein expression and the subsequent lack of serological assays have hampered TTV seroprevalence studies. Moreover, the antigenic relationships of different TTVs and their specific seroprevalences in humans remain unknown. For five TTV strains belonging to different species of four genogroups - we developed, using recombinant glutathione S-transferase (GST)-fused TTV ORF2 proteins, glutathione GST capture enzyme immunoassays (EIAs) detecting antibodies towards conformational epitopes. We then analysed serum samples from 178 healthy adults and 108 children; IgG reactivities were observed either towards a single strain or towards multiple strains, which pointed to antigenic distinction of TTV species. The overall seroprevalence for the five TTVs peaked at 43% (18 of 42) in children 2-4 years of age, subsequently declined, and again reached 42 % (74 of 178) among adults. TTV6 species-specific IgG predominated in children, whereas that for TTV13 predominated in adults. During a 3 year follow-up of the same children, both species-specific seroconversions and seroreversions occurred. This is the first EIA-based study of different TTVs, providing a new approach for seroepidemiology and diagnosis of TTV infections. Our data suggest that different TTVs in humans may differ in antiviral antibody profiles, infection patterns and epidemiology.