Genetic variation among vegetative compatibility groups of Fusarium oxysporum f. sp. cubense analyzed by DNA fingerprinting
Genetic variation among vegetative compatibility groups of Fusarium oxysporum f. sp. cubense analyzed by DNA fingerprinting
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DOI：
10.1094/phyto.1998.88.12.1283
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发表时间：
1998-12-01
期刊：
影响因子：
3.2
通讯作者：
Buddenhagen, IW
中科院分区：
文献类型：
--
作者：
Bentley, S;Pegg, KG;Buddenhagen, IW
全球 208 个尖孢镰刀菌 f 分离株的遗传变异。 sp。 cubense 代表生理种族 1、2、3 和 4 以及 20 个报告的营养相容性群体 (VCG)，使用改良的 DNA 扩增指纹分析进行分析。还鉴定了 133 个分离株，它们不属于任何已报道的尖孢镰刀菌 VCG。 sp。 cubense 包括从 Heliconia 物种中分离出来的第 3 种菌株，以及从生长在马来西亚半岛丛林中的有症状的野生香蕉物种中分离出来的菌株。 DNA 指纹图谱通常是 VCG 特异性的，与地理或宿主来源无关。 F：尖孢菌 f 中总共鉴定出 33 种不同的基因型。 sp。立方体；在属于 20 个报告的 VCG 的分离株中区分出 19 个基因型，在不属于任何现有 VCG 的分离株中鉴定出 14 个新基因型。 DNA 指纹分析还可以区分 F. oxysporum f 内的九个克隆谱系。 sp。古巴。其中五个谱系各自包含大量密切相关的 VCG 和基因型，其余四个谱系各自包含一个基因型。 F. oxysporum f. 几个谱系的遗传多样性和地理分布。 sp。 cubense 表明它们与亚洲的可食用香蕉及其野生二倍体祖先共同进化。对野生病理系统分离株的 DNA 指纹分析为共同进化假说提供了进一步的证据。 F. oxysporum f. 四个谱系的遗传隔离和有限的地理分布。 sp。 cubense表明病原体也是独立产生的，无论是在宿主起源中心的内部还是外部。
Genetic variation within a worldwide collection of 208 isolates of Fusarium oxysporum f. sp. cubense, representing physiological races 1, 2, 3, and 4 and the 20 reported vegetative compatibility groups (VCGs), was analyzed using modified DNA amplification fingerprinting. Also characterized were 133 isolates that did not belong to any of the reported VCGs of F. oxysporum f. sp. cubense including race 3 isolates from a Heliconia species and isolates from a symptomatic wild banana species growing in the jungle in peninsular Malaysia. The DNA fingerprint patterns were generally VCG specific, irrespective of geographic or host origin. A total of 33 different genotypes were identified within F: oxysporum f. sp. cubense; 19 genotypes were distinguished among the isolates that belonged to the 20 reported VCGs, and 14 new genotypes were identified among the isolates that did not belong to any of the existing VCGs. DNA fingerprinting analysis also allowed differentiation of nine clonal lineages within F. oxysporum f. sp. cubense. Five of these lineages each contained numerous closely related VCGs and genotypes, and the remaining four lineages each contained a single genotype. The genetic diversity and geographic distribution of several of these lineages of F. oxysporum f. sp. cubense suggests that they have coevolved with edible bananas and their wild diploid progenitors in Asia. DNA fingerprinting analysis of isolates from the wild pathosystem provides further evidence for the coevolution hypothesis. The genetic isolation and limited geographic distribution of four of the lineages of F. oxysporum f. sp. cubense suggests that the pathogen has also arisen independently, both within and outside of the center of origin of the host.