Micellar electrokinetic chromatography systems for the separation of mixtures of charged and uncharged compounds.
Micellar electrokinetic chromatography systems for the separation of mixtures of charged and uncharged compounds.
复制标题
用于分离带电和不带电化合物混合物的胶束电动色谱系统。
DOI：
--
复制
发表时间：
2012
影响因子：
3.1
通讯作者：
M. Fillet
中科院分区：
文献类型：
--
作者：
C. Lamalle;A. Servais;I. Fradi;J. Crommen;M. Fillet
在这项研究中，带电和不带电的分析物的迁移行为进行了研究，在不同的条件下。在中性、碱性和酸性pH值（7.5、11和2.2）下，使用含有不同十二烷基硫酸钠（SDS）浓度（0-90 mM）和乙腈（ACN）比例（0-75%）的背景电解质，测量阳离子、阴离子和中性物质的有效迁移率-胶束影响下的电泳迁移率。SDS浓度和ACN比例对模型化合物的有效迁移率和迁移级数有很大的影响。尽管SDS胶束优选与中性物和阳离子相互作用，但疏水键也可以与阴离子发生。阳离子、阴离子和中性离子的迁移行为有很大的不同，通过适当调节SDS浓度和ACN比例，可以大大提高方法的选择性。这些观察结果证实了使用胶束电动色谱不仅用于分离中性物质，而且用于分析带电化合物的兴趣。
In this study, the migration behavior of charged and uncharged analytes was investigated under different conditions. Effective mobilities - electrophoretic mobilities under the influence of micelles - of cations, anions, and neutrals were measured at neutral, basic, and acidic pH (7.5, 11, and 2.2) using background electrolytes containing different sodium dodecyl sulfate (SDS) concentrations (0-90 mM) and acetonitrile (ACN) proportions (0-75%). SDS concentration and ACN proportion were found to have a tremendous effect on the effective mobilities and migration order of the model compounds. Although the SDS micelles preferably interact with neutrals and cations, hydrophobic bonds can also occur with anions. Cations, anions, and neutrals having rather different migration behaviors, it is possible to considerably enhance the selectivity of the method by adjusting properly the SDS concentration and the ACN proportion. These observations confirm the interest of using micellar electrokinetic chromatography not only for the separation of neutral substances but also to analyze charged compounds.