Peculiar immunobiology of bone marrow allografts. I. Graft rejection by irradiated responder mice.
Peculiar immunobiology of bone marrow allografts. I. Graft rejection by irradiated responder mice.
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DOI：
10.1084/jem.134.1.83
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发表时间：
1971-07-01
期刊：
影响因子：
--
通讯作者：
Bennett M
中科院分区：
文献类型：
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作者：
Cudkowicz G;Bennett M
在一次致命的x射线照射后，小鼠能够排斥h -2不相容的骨髓移植。排斥反应开始于移植后18-24小时，并于96小时结束。这种类型的同种异体移植物反应性的成熟直到生命的第22天才发生。在成年小鼠中，对同种异体骨髓移植的抵抗力可以通过环磷酰胺或小棒状杆菌的死培养物来减弱，但不能通过异源抗胸腺细胞血清来减弱。移植前7或14天暴露于x射线也会削弱抵抗力。即使宿主和供体之间的H-2差异相同，小鼠抵抗同种异体移植物的能力也存在相当大的品系间差异。虽然H-2不相容是抵抗的必要前提，但其他遗传因素可能通过控制识别来影响骨髓移植的结果。调节基因是决定特异性的，抗性或应答状态的等位基因似乎占主导地位。应答表型是由造血细胞而不是由环境表达的。因此，在转移骨髓细胞而不是血清后，抗性被赋予其他易感小鼠。骨髓移植排斥反应效应细胞的产生和分化与胸腺无关。总之，同种异体骨髓移植引起一种特殊的移植反应，以前未知，在辐照小鼠。这种反应的特点是缺乏宿主淋巴细胞的增殖，组织特异性，胸腺独立性，受遗传因素的调节，显然不影响其他移植物的命运。
Mice are capable of rejecting H-2-incompatible bone marrow grafts after a single lethal exposure to X-rays. The onset of rejection begins 18–24 hr after transplantation and is completed by 96 hr. Maturation of this type of allograft reactivity does not occur until the 22nd day of life. In adult mice, the resistance to marrow allografts can be weakened by administration of cyclophosphamide or dead cultures of Corynebacterium parvum, but not heterologous anti-thymocyte serum. Sublethal exposures to X-rays 7 or 14 days before transplantation also weaken resistance. There is considerable interstrain variation in the ability of mice to resist allografts, even when H-2 differences between hosts and donor are kept identical. Although H-2 incompatibility is a necessary prerequisite for resistance, additional genetic factors influence the outcome of marrow allografts, presumably by controlling recognition. The regulator genes are determinant specific and the alleles for resistance or responder status appear to be dominant. The responder phenotype is expressed by hemopoietic cells and not by the environment. Accordingly, resistance is conferred to otherwise susceptible mice upon transfer of bone marrow cells but not of serum. The production and differentiation of effector cells for marrow graft rejection are thymus independent. In conclusion, bone marrow allografts elicit a particular transplantation reaction, previously unknown, in irradiated mice. Peculiar features of this reaction are the lack of proliferation of host lymphoid cells, tissue specificity, thymus independence, and regulation by genetic factors which apparently do not affect the fate of other grafts.