The GPI-anchored gas and Crh families are fungal antigens
The GPI-anchored gas and Crh families are fungal antigens
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DOI：
10.1002/yea.1480
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发表时间：
2007-04-01
期刊：
影响因子：
2.6
通讯作者：
Latge, Jean Paul
中科院分区：
文献类型：
--
作者：
Arroyo, Javier;Sarfati, Jaqueline;Latge, Jean Paul
细胞壁是真菌与其细胞外环境之间的第一个界面。最近已鉴定出参与细胞壁多糖网络的形成和动态重塑的糖基转移酶。最具特征的属于 Gas 家族，它可以延长 β(1,3)-葡聚糖，而 Crb 家族则参与几丁质与 β(1,6) 葡聚糖的交联。所有这些蛋白质都带有糖基磷脂酰肌醇 (GPI) 锚。在这项工作中，我们证明来自酿酒酵母的重组可溶形式的 Gas1-5 和 Crh1p 以及来自烟曲霉的直系同源蛋白 Gel1-Gel2 和 Crf1 可以被曲霉菌或念珠菌感染患者血清中存在的抗体特异性识别。针对重组 Gas/Gel 和 Crh/Crf 蛋白的抗体滴度定量将曲霉瘤和念珠菌病患者与未感染个体分开。曲霉菌病和念珠菌病患者对 Gas/Gel 和 Crh/Crf 蛋白的抗体反应之间存在交叉反应。这些结果表明，GPI 锚定的交联酶是细胞壁的相关免疫反应成分，可能在人类真菌感染过程中发挥作用。版权所有 (c) 2007 John Wiley & Sons, Ltd.
The cell wall is the first interface between a fungus and its extracelullar environment. Glycosyltransferases involved in the formation and dynamic remodelling of the polysaccharide network of the cell wall have recently been identified. The best characterized ones belong to the Gas family, which elongates beta(1,3)-glucans, and to the Crb family, which are involved in the cross-linking of chitin to beta(1,6)glucan. All these proteins carry a glycosylphosphatidylinositol (GPI) anchor. In this work, we show that recombinant soluble forms of Gas1-5 and Crh1p from Saccharomyces cerevisiae and their orthologous proteins Gel1-Gel2 and Crf1 from Aspergillus fumigatus are specifically recognized by antibodies present in the sera of patients with Aspergillus or Candida infections. Quantification of the antibody titres against recombinant Gas/Gel and Crh/Crf proteins separated aspergilloma and candidiasis patients from non-infected individuals. Cross-reactivity was seen between the antibody response of patients with aspergillosis and candidiasis towards the Gas/Gel and Crh/Crf proteins. These results suggest that GPI-anchored cross-linking enzymes are relevant immunologically reactive constituents of the cell wall that may play a role during human fungal infections. Copyright (c) 2007 John Wiley & Sons, Ltd.