Pressure-induced crystal memory effect of spider silk proteins.
Pressure-induced crystal memory effect of spider silk proteins.
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DOI：
10.1021/jp811461b
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发表时间：
2009-04
期刊：
影响因子：
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通讯作者：
Hongsen Peng;Shaobing Zhou;Jing Jiang;Tao Guo;Xiaotong Zheng;Xiongjun Yu
中科院分区：
文献类型：
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作者：
Hongsen Peng;Shaobing Zhou;Jing Jiang;Tao Guo;Xiaotong Zheng;Xiongjun Yu
蜘蛛丝是一种新型材料，具有高强度和韧性，重量轻，使其在高性能纤维和生物医学应用中具有吸引力。随着科学技术的发展，蜘蛛丝蛋白的氨基酸序列和分子结构逐渐被人们所了解。然而，其结构与功能的关系一直是材料科学家们研究的一个难题，本研究以蜘蛛丝蛋白为材料，研究了不同加工工艺对蜘蛛丝蛋白晶体结构的影响。虽然生物大分子的结晶是非常困难和复杂的，但我们确实观察到了静电纺丝蜘蛛丝蛋白在其固态下经外部高压处理后的晶体。本研究为蛋白质晶体的制备提供了一种简单有效的方法。最重要的是，这是我们第一次发现蜘蛛丝蛋白作为静电纺丝的结果，在高压刺激下具有结晶记忆特性，这将进一步扩展其特定特性的列表。这一发现也将引导我们深入研究它，并扩大我们对其他生物蛋白质的研究。
Spider silk is a novel material with high strength and toughness and is lightweight, making it attractive for high-performance fiber and biomedical applications. The amino acid sequence and molecular architecture of spider silk protein are becoming known gradually with science and technology development. However, its relationship of structure and function is a puzzle which attracts materials scientists to unveil it. In this study we investigated the changes of the crystal structure as a result of different processing techniques to spider silk protein. Although the crystallization of biomacromolecules is very difficult and complex, we did observe the crystal of electrospun spider silk protein treated by external high pressure in its solid state. This study also provides us a simple and effective method to prepare protein crystals. It is most important that this is the first time that we found spider silk protein as a result of electrospinning that had a crystallization memory property when it was stimulated by a high pressure, which will further extend the list of its particular properties. This finding will also lead us to study it deeply and widen our research on proteins of other organisms.