Histological responses of cutaneous vascular lesions following photodynamic therapy with talaporfin sodium: a chicken comb model.
Histological responses of cutaneous vascular lesions following photodynamic therapy with talaporfin sodium: a chicken comb model.
复制标题
他拉泊芬钠光动力治疗后皮肤血管病变的组织学反应：鸡冠模型。
DOI：
10.2302/kjm.58.176
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
K. Kishi
中科院分区：
文献类型：
--
作者：
T. Ohshiro;T. Nakajima;H. Ogata;K. Kishi
背景
单-L-乙酰二氢卟酚e6（Talaporfin sodium）是一种新型光敏剂，目前正与664 nm激光照射联合用于各种恶性肿瘤的光动力学治疗。他拉泊芬钠的一个有趣的特性是，注射该药剂后皮肤光敏性比任何其他现有的光敏剂消失得更快。本研究以他拉泊芬钠光敏化后的毛细血管畸形模型为例，检查了鸡梳子辐射后发生的血管事件。
材料和方法
将他拉泊芬钠的单次静脉推注给予鸡，并用664 nm可见红色激光照射每只动物的梳子的1 cm直径区域。在光动力疗法后7-14天记录鸡冠的大体变化。对于组织学检查，分析HE、PTAH和Azan染色切片。
结果
所有处理过的鸡冠在光动力治疗后都变白了。显微镜检查显示没有红细胞，血管腔消失，留下正常的覆盖表皮完全完整。在选择性破坏目标区域毛细血管的同时，观察到炎症细胞中度侵入和基质轻微增加。
结论
在鸡冠模型中，使用他拉泊芬钠的光动力疗法有效地实现了微血管的选择性破坏，同时保持表皮完整。我们的研究结果有力地表明，他拉泊芬钠光动力疗法可能是一种可行的方法来治疗皮肤富血管病变。
BACKGROUND
Mono-L-aspartyl chlorin e6 (Talaporfin sodium) is a novel photosensitizer, and is currently being used in photodynamic therapy for various malignant tumors in combination with irradiation with a 664 nm laser. An interesting characteristic of Talaporfin sodium is that the skin photosensitivity after injection of this agent disappears faster than any other existing photosensitizers. This study examined the vascular events that occurred postirradiation in the chicken comb as a capillary malformation model after photosensitization with Talaporfin sodium.
MATERIALS AND METHODS
A single intravenous bolus injections of Talaporfin sodium was administered to the chickens, and a 1 cm diameter area of the comb of each animal was irradiated with a 664 nm visible red laser. The gross changes in the chicken combs were recorded for 7-14 days after photodynamic therapy. For the histological examination, HE, PTAH and Azan stained sections were analyzed.
RESULTS
All treated chicken combs had blanched after photodynamic therapy. Microscopy demonstrated an absence of erythrocytes and the vessel lumina were obliterated, leaving the normal overlying epidermis completely intact. Concomitantly with selective destruction of the capillaries in the target area, moderate invasion of inflammatory cells and a slight increase in the stroma were observed.
CONCLUSIONS
In the chicken comb model, photodynamic therapy with Talaporfin sodium effectively achieved selective destruction of the microvasculature while leaving the epidermis intact. Our results strongly suggest that photodynamic therapy with Talaporfin sodium could be a feasible method to treat dermal hypervascular lesions.
使用单-L-天冬氨酰二氢卟酚 e6 的光动力疗法对血管收缩、血管渗漏和肿瘤反应的影响。
影响因子：
11.2
作者：
McMahon,KS;Wieman,TJ;Moore,PH;Fingar,VH
通讯作者：
Fingar,VH
影响因子：
10.3
作者：
NELSON, JS;LIAW, LH;BERNS, MW
通讯作者：
BERNS, MW