MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer
MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer
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MYBL2 是一种促进胆囊癌细胞增殖的潜在预后标志物
DOI：
10.1159/000475454
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发表时间：
2017-01-01
影响因子：
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通讯作者：
Liu, Ying-Bin
中科院分区：
文献类型：
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作者：
Liang, Hai-Bin;Cao, Yang;Liu, Ying-Bin
Background: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 expression levels were measured in GBC and cholecystitis assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl- 2'-deoxyuridine (EdU) in nude mice. Results: MYBL2 expression was increased in GBC tissues and associated with histological differentiation, tumour invasion, clinical stage and unfavourable overall survival in GBC patients. The downregulation of MYBL2 expression resulted in the inhibition of GBC cell proliferation, and DNA replication in vitro, and the growth of xenografted tumours in nude mice. Conversely, MYBL2 overexpression resulted in the opposite effects. Conclusions: MYBL2 overexpression promotes GBC cell proliferation through the regulation of the cell cycle at the S and G2/M phase transitions. Thus, MYBL2 could serve as a potential prognostic and therapeutic biomarker in GBC patients. (C) 2017 The Author(s) Published by S. Karger AG, Basel.
