Analysis of endosperm development of allotriploid x diploid/tetraploid crosses in Lilium
Analysis of endosperm development of allotriploid x diploid/tetraploid crosses in Lilium
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百合异源三倍体与二倍体/四倍体杂交的胚乳发育分析
DOI：
10.1007/s10681-011-0609-5
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发表时间：
2012-04-01
期刊：
影响因子：
1.9
通讯作者：
Zhou, Guixue
中科院分区：
文献类型：
--
作者：
Zhou, Shujun;Li, Kehu;Zhou, Guixue
百合是贝母属植物。三倍体百合，无论其雄性不育性如何，都可以用作母本与适当的雄性杂交，这与通常无籽的其他三倍体蓼型植物相反。但迄今为止，其原因却很少受到关注。在这里，我们在百合中进行了异源三倍体 x 二倍体/四倍体杂交。分析了 LAA x AA/AAAA 杂交的胚乳和后代的倍性水平和基因组组成。一些LAA x AA/AAAA胚乳的胚乳发育良好，但LAA x LL/OO胚乳很少或没有发育。 LAA x AA、LAA x AAAA、LAA x LL、LAA x OO 的胚乳基因组组成理论上分别为 5A + 2L、6A + 2L、4A + 3L、4A + 2L + O。基因组原位杂交表明LAA x AA/AAAA的后代为非整倍体。根据实验结果和分析，我们的假设是，五个相同的胚乳基因组对于百合的三倍体 x 二倍体/四倍体杂交的发育至关重要。该假设可以解释百合 3x x 2x/4x 杂交的成功或失败，对于百合育种者来说非常重要，因为他们在生产这种经济上重要的园艺作物的新品种时需要知道成功的可能性。
Lilium are Fritillaria-type plants. Triploid lilies, regardless of their male sterility, can be used as female parents to cross with appropriate males, in contrast to other triploid Polygonum-type plants, which are usually seedless. Up to now, however, little attention has been paid to the reason. Here we made allotriploid x diploid/tetraploid crosses in Lilium. Endosperm and progenies of LAA x AA/AAAA crosses were analyzed for ploidy level and genome composition. The endosperm of some LAA x AA/AAAA ovules developed well but there was little or no development of LAA x LL/OO endosperm. The endosperm genome composition of LAA x AA, LAA x AAAA, LAA x LL, LAA x OO are theoretically derived as 5A + 2L, 6A + 2L, 4A + 3L, 4A + 2L + O, respectively. Genomic in situ hybridization showed that the progenies of LAA x AA/AAAA were aneuploid. Based on the experimental results and analyses, our hypothesis is that five same genomes of endosperms are essential for its development in triploid x diploid/tetraploid crosses of Lilium. The hypothesis can explain the success or failure of 3x x 2x/4x crosses in Lilium and is of importance for lily breeders who need to know the likelihood of success when producing new cultivars of this economically important horticultural crop.