RNA interference of ace1 and ace2 in Chilo suppressalis reveals their different contributions to motor ability and larval growth
RNA interference of ace1 and ace2 in Chilo suppressalis reveals their different contributions to motor ability and larval growth
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DOI：
10.1111/j.1365-2583.2011.01081.x
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发表时间：
2011-08-01
影响因子：
2.6
通讯作者：
Li, F.
中科院分区：
文献类型：
--
作者：
Hui, X. -M.;Yang, L. -W.;Li, F.
乙酰胆碱酯酶（Acetylcholinesterase，AChE，EC 3.1.1.7）是终止突触传递的关键酶。我们敲低表达的Csace 1或Csace 2使用化学合成的小干扰RNA（siRNA）的设计从不同的区域。两种ace基因的mRNA丰度降低至对照水平的50-70%。酶活性下降到40- 70%。Csace 1或Csace 2的沉默导致类似于25%的死亡率。敲低Csace 1对幼虫生长抑制有重大影响，并导致幼虫体重和长度减少，畸形和运动障碍，而沉默Csace 2只有轻微的影响。这些结果表明，AChE-1和AChE-2在维持该虫生命中具有重要作用，并提示AChE-1可能在调节幼虫生长和运动能力方面具有非典型功能。
Acetylcholinesterase (AChE, EC 3.1.1.7) is a key enzyme in terminating synaptic transmission. We knocked down the expression of Csace1 or Csace2 using chemically synthesized small interfering RNAs (siRNAs) designed from divergent regions. The mRNA abundance of the two ace genes was reduced to 50-70% of control levels. The enzyme activities were decreased to 40-70%. Silencing of Csace1 or Csace2 resulted in a similar to 25% mortality rate. Knockdown of Csace1 had major effects on larval growth inhibition and resulted in reduced larval weight and length, malformation and motor disability, whereas silencing of Csace2 had only minor effects. These results suggested that both AChE-1 and AChE-2 have important roles in maintaining life in this insect and indicated that AChE-1 might have nontypical functions in regulating larval growth and motor ability.