Antibacterial properties of human amnion and chorion in vitro
Antibacterial properties of human amnion and chorion in vitro
复制标题
DOI：
10.1016/s0301-2115(00)00345-6
复制
发表时间：
2001-02-01
期刊：
影响因子：
--
通讯作者：
Madsen, H
中科院分区：
文献类型：
--
作者：
Kjaergaard, N;Hein, M;Madsen, H
目标。本研究的目的是探讨羊膜抗绒毛膜在体外对细菌生长的直接影响，包括抗菌谱。取自13例足月择期剖宫产的健康妇女，在无菌条件下获得绒毛羊膜。我也是。取自10例足月自然分娩的健康妇女的绒毛羊膜。对5株B组溶血性链球菌(GBS)和1株临床分离的下列菌种或菌群进行了检测：A组溶血性链球菌、A组金黄色葡萄球菌。腐生葡萄球菌，粪肠球菌，大肠埃希菌。铜绿假单胞菌、醋酸钙不动杆菌和乳杆菌。细菌培养基组分为(1)血琼脂培养基组：(2)液体培养用透明琼脂组培基；(3)营养肉汤组培基。结果：胎膜对多种细菌均有抑制作用。在琼脂培养和远处悬浮培养(两项研究中都有8名女性的胎膜)的实验中，获得了一致的结果。在液体培养的实验中(7名女性，只有绒毛膜表现出轻微的抑制作用。对所有菌株均有抑制作用，但绒毛膜和羊膜对金黄色葡萄球菌和腐生葡萄球菌的抑制作用最明显。结论：这项研究证实了胎膜油对多种细菌的抑制作用(C)2001爱思唯尔科学爱尔兰有限公司。保留所有权利。
Objective. The purpose of the present study was to explore the direct effects of amnion anti chorion on bacterial growth in vitro including the antibacterial spectrum. Chorioamniotic membranes were obtained under sterile conditions from 13 healthy women undergoing elective cesarean section at term. Likewise. chorioamniotic membranes were obtained from 10 healthy women with spontaneous vaginal delivery at term. Five strains of Hemolytic streptococci group B (GBS) were tested and one clinical isolate of the following species or bacterial groups: Hemolytic streptococcus group A. Staphylococcus aureus. Staphylococcus saprophyticus, Enterococcus faecalis, Escherichia coli. Pseudomonas aeruginosa, Acinetobacter calcoaceticus and Lactobacillus species. Bacteriological media included (1) blood-agar medium: (2) a transparent agar medium for submerged cultures; and (3) a nutrient broth medium. Results: An inhibitory effect of fetal membranes against a range of bacteria was found. Consistent results were obtained in experiments with cultures on agar and cultures suspended in afar (membranes from eight women in both studies). In experiments with liquid cultures (seven women, only chorion showed a marginal inhibitory effect. All strains were inhibited, but the most pronounced inhibition was obtained for streptococcus group A. S. aureus and S. saprophyticus by both chorion and amnion. Conclusion: This study demonstrated an inhibitory effect of the fetal membranes oil a diverse panel of bacteria (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.