Effect of a formulation of Bacillus firmus on root-knot nematode Meloidogyne incognita infestation and the growth of tomato plants in the greenhouse and nursery
Effect of a formulation of Bacillus firmus on root-knot nematode Meloidogyne incognita infestation and the growth of tomato plants in the greenhouse and nursery
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DOI：
10.1016/j.jip.2008.11.004
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发表时间：
2009-02-01
影响因子：
3.4
通讯作者：
Sakhuja, P. K.
中科院分区：
文献类型：
--
作者：
Terefe, Metasebia;Tefera, Tadele;Sakhuja, P. K.
在实验室、温室和田间条件下，测定了商品可湿性粉剂(BioNem)对番茄根结线虫病的防治效果。在实验室试验中，浓度为0.5%、1%、1.5%和2%的BioNem水悬浮液处理24天后，卵孵化率从98%下降到100%。用2.5%和3%的BioNem处理第二期幼虫，在处理后24 h，对活动能力的抑制程度达到100%。在温室试验中，在每盆8g(1200cc土壤)下种植番茄幼苗时，应用BioNem可减少91%的虫害形成，最终线虫种群减少76%，产卵量减少45%。因此，施药50天后，株高和生物量分别比未处理对照增加了71%和50%。生物纳米管的用量为16g/盆，对植物有一定的药害作用。在田间试验中，在处理后45d，200和400 kg ha(-1)的BioNem能有效地减少虫害数量(75-84%)，增加地上部高度(29-31%)和重量(20-24%)。结果表明，固氮菌是防治番茄盆栽南方根结线虫的一种很有前途的微生物。(C)2008 Elsevier Inc.保留所有权利。
Bacillus firmus, commercial WP formulation (BioNem) was evaluated against the root-knot nematode Meloidogyne incognita in a laboratory, greenhouse and under field conditions on tomato plants. In the laboratory tests, an aqueous suspension of BioNem at 0.5%, 1%, 1.5% and 2% concentration reduced egg hatching from 98% to 100%, 24-days after treatment. Treatment of second-stage juveniles with 2.5% and 3% concentration of BioNem, caused 100% inhibition of mobility, 24 h after treatment. In the green house trials, BioNem applied at 8 g/pot (1200 cc soil) planted with a tomato seedlings reduced gall formation by 91%, final nematode populations by 76% and the number of eggs by 45%. Consequently, plant height and biomass was increased by 71% and 50%, respectively, compared to the untreated control, 50-days after treatment application. Application of BioNem at 16 g/pot was phytotoxic to plants. In the field trails, BioNem applied at 200 and 400 kg ha(-1) was effective in reducing the number of galls (75-84%), and increased shoot height (29-31%) and weight (20-24%) over the untreated control, 45-days after treatment. Our results indicate that B. firmus is a promising microorganism for the biological control of M. incognita in tomato pots. (C) 2008 Elsevier Inc. All rights reserved.