FAST AND EASY DETECTION OF MOUSE SEX CHIMERAS USING ELECTROPHORETIC POLYMORPHISM OF PHOSPHOGLYCERATE KINASE-1, AN X-CHROMOSOME-LINKED ENZYME
FAST AND EASY DETECTION OF MOUSE SEX CHIMERAS USING ELECTROPHORETIC POLYMORPHISM OF PHOSPHOGLYCERATE KINASE-1, AN X-CHROMOSOME-LINKED ENZYME
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DOI：
10.1095/biolreprod39.4.923
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发表时间：
1988-11-01
影响因子：
3.6
通讯作者：
KITAMURA, Y
中科院分区：
文献类型：
--
作者：
NAKAYAMA, H;KURODA, H;KITAMURA, Y
About half of the chimeras produced by aggregation of two mouse embyros are sex chimeras composed of both XX and XY cells. We developed a fast and easy method to identify sex chimeras by using electrophoretic bimorphism of an X-linked enzyme, phosphoglycerate kinase-1 (PGK-1), as a marker. When embryos resulting from the crossing of a Pgk-1b/Pgk-1b female and a Pgk-1a/Y male are aggregated, the genotype of sex chimeras is Pgk-1b/Pgk-1a.tautm.Pgk-1b/Y. Most of these were identifiable from the PGK-1 electrophoretic pattern of blood cells (i.e., AB type) and the appearance of genitalia (male type or apparently abnormal). Genotypes of functional sperm in the testes of the male-type sex chimeras were also identifiable from the PGK-1 electrophoretic pattern of progenies. Examination of gonads of the sex chimeras revealed that a considerable proportion was hermaphroditic. With this method, reasonable numbers of male-type sex chimeras and hermaphrodites may be selected and used as materal for investigating sexual differentiation.
