Detection of trabecular bone microdamage by micro-computed tomography.
Detection of trabecular bone microdamage by micro-computed tomography.
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DOI：
10.1016/j.jbiomech.2007.05.009
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发表时间：
2007
影响因子：
2.4
通讯作者：
Xiang-Shuo Wang;Daniel B Masse;H. Leng;Kevin P Hess;R. Ross;R. Roeder;G. Niebur
中科院分区：
文献类型：
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作者：
Xiang-Shuo Wang;Daniel B Masse;H. Leng;Kevin P Hess;R. Ross;R. Roeder;G. Niebur
微损伤是骨质量的重要组成部分，影响骨重塑。改进的评估微损伤的技术无需进行组织学切片，通过测量小梁微结构内损伤的空间分布，可以深入了解微损伤在小梁骨强度中的作用。制备柱状骨小梁标本19例，分为两组。ⅰ组试样损伤至3%压应变，用BaSO4标记。II组未装载，但用BaSO4标记。显微计算机断层扫描（Micro-CT）图像分辨率为10μm。比较两组间治疗后骨组织的中位强度。阈值法也用于测量微ct扫描中的损伤面积。加载组的组织学测量损伤面积分数、CT中位数强度、micro-CT测量损伤面积分数均高于卸载组，说明micro-CT图像可以区分损伤标本组和卸载标本。组织学测量的损伤面积分数与micro-CT测量的损伤面积分数和骨CT中位强度呈正相关，说明micro-CT图像可以检测骨小梁的微损伤，具有足够的准确性，可以区分不同样品的损伤程度。该技术提供了一种非侵入性评估骨小梁试件中微损伤三维分布的方法，并可用于深入了解骨小梁结构在微损伤形成中的作用。
Microdamage is an important component of bone quality and affects bone remodeling. Improved techniques to assess microdamage without the need for histological sectioning would provide insight into the role of microdamage in trabecular bone strength by allowing the spatial distribution of damage within the trabecular microstructure to be measured. Nineteen cylindrical trabecular bone specimens were prepared and assigned to two groups. The specimens in group I were damaged to 3% compressive strain and labeled with BaSO4. Group II was not loaded, but was labeled with BaSO4. Micro-computed tomography (Micro-CT) images of the specimens were obtained at 10μm resolution. The median intensity of the treated bone tissue was compared between groups. Thresholding was also used to measure the damaged area fraction in the micro-CT scans. The histologically measured damaged area fraction, the median CT intensity, and the micro-CT measured damaged area fraction were all higher in the loaded group than in the unloaded group, indicating that the micro-CT images could differentiate the damaged specimen group from the unloaded specimens. The histologically measured damaged area fraction was positively correlated with the micro-CT measured damaged area fraction and with the median CT intensity of the bone, indicating that the micro-CT images can detect microdamage in trabecular bone with sufficient accuracy to differentiate damage levels between samples. This technique provides a means to non-invasively assess the three-dimensional distribution of microdamage within trabecular bone test specimens and could be used to gain insight into the role of trabecular architecture in microdamage formation.