lncRNA SLCO4A1-AS1 promotes growth and invasion of bladder cancer through sponging miR-335-5p to upregulate OCT4
lncRNA SLCO4A1-AS1 promotes growth and invasion of bladder cancer through sponging miR-335-5p to upregulate OCT4
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DOI：
10.2147/ott.s191740
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发表时间：
2019-01-01
影响因子：
4
通讯作者：
Men, Tongyi
中科院分区：
文献类型：
--
作者：
Yang, Yu;Wang, Feng;Men, Tongyi
背景：膀胱癌是泌尿系统最常见的癌症类型之一。近年来，LncRNAs在不列颠哥伦比亚省的重要性已被认识到。SLCO4A1-AS1是结直肠癌的癌基因。然而，SLCO4A1-AS1在BC中的作用尚不清楚。材料和方法：采用qRT-PC R方法分析SLCO4A1-AS1在BC组织中的表达水平，用CCK8法检测SLCO4A1-AS1基因敲除对BC细胞增殖的影响。Transwell法检测SLCO4A1-AS1在细胞迁移和侵袭中的作用。结果：SLCO4A1-AS1在结直肠癌组织中的表达明显高于癌旁正常组织。此外，SLCO4A1-AS1水平与BC的分期和转移呈正相关。SLCO4A1-AS1表达上调提示BC患者预后不良。SLCO4A1-AS1基因的敲除下调了EJ和T24细胞的增殖、迁移和侵袭。此外，SLCO4A1-AS1的缺失阻碍了BC在体内的生长。机制研究表明SLCO4A1-AS1是miR-335-5p的海绵，miR-335-5p调控OCT4的表达。结论：SLCO4A1-AS1的高表达与BC的进展有关，SLCO4A1-AS1通过miR-335-5p/OCT4轴促进BC细胞的恶性表型。
Background: Bladder cancer (BC) is among the most frequently occurring cancer types in the urinary system. In recent years, the importance of lncRNAs in BC has been acknowledged. SLCO4A1-AS1 is an oncogene in colorectal cancer. However, the role of SLCO4A1-AS1 in BC remains unknown.Materials and methods: The expression levels of SLCO4A1-AS1 in BC tissues were analyzed by qRT-PC R. The effects of SLCO4A1-AS1 knockdown on proliferation were determined by CCK8 assay. Transwell assay was used to evaluate the role of SLCO4A1-AS1 on migration and invasion. Furthermore, xenograft assay was utilized to test the effect of SLCO4A1-AS1 on BC growth in vivo.Results: SLCO4A1-AS1 expression was more upregulated in BC tissues than in adjacent normal tissues. Moreover, SLCO4A1-AS1 level was positively correlated with the advanced stage and metastasis in BC. The upregulation of SLCO4A1-AS1 indicates poor prognosis in BC patients. The knockdown of SLCO4A1-AS1 downregulated the proliferation, migration, and invasion of EJ and T24 cells in vitro. In addition, the loss of SLCO4A1 -AS1 prevented BC growth in vivo. Mechanistic investigation showed that SLCO4A1-AS1 was the sponge for miR-335-5p, and miR-335-5p modulated OCT4 expression.Conclusion: High SLCO4A1-AS1 expression level was associated with the progression of BC, and SLCO4A1-AS1 promoted the malignant phenotypes of BC cells through the miR-335-5p/OCT4 axis.