Effects of supplemental dietary bile acids on growth, liver function and immunity of juvenile largemouth bass (Micropterus salmoides) fed high-starch diet
Effects of supplemental dietary bile acids on growth, liver function and immunity of juvenile largemouth bass (Micropterus salmoides) fed high-starch diet
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膳食补充胆汁酸对高淀粉饲料喂养的大口黑鲈幼鱼生长、肝功能和免疫力的影响
DOI：
10.1016/j.fsi.2019.12.087
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发表时间：
2020-02-01
影响因子：
4.7
通讯作者：
Lin, Shi-Mei
中科院分区：
文献类型：
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作者：
Guo, Jia-Ling;Kuang, Wen-Ming;Lin, Shi-Mei
研究了高淀粉饲料中添加胆汁酸对大口黑鲈生长、肝功能和免疫功能的影响。试验设3种等氮等能半纯化日粮，LS为低淀粉日粮（5%），HS为高淀粉日粮（19%），SB为高淀粉日粮加BA（350 mg/kg）。在初始重量为23.69 ± 0.13 g的大口黑鲈中进行8周的喂养试验。结果表明，LS和SB处理的增重和蛋白质效率比（PER）显著高于HS处理。与HS组相比，SB组肝脏超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性显著升高，丙二醛（MDA）含量显著降低。SB组的丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）活性及血浆葡萄糖含量显著低于HS组，而血浆甘油三酯（TG）和总胆固醇（TC）含量显著高于HS组。SB组血浆免疫球蛋白、溶菌酶活性及白细胞计数均显著高于HS组。石蜡切片结果显示SB组肝组织病理学改变较HS组明显减轻。结果表明，胆汁酸能显著提高马立克次氏体的生长性能，增强肝功能和免疫能力，缓解应激反应。鲑科鱼类饲喂高淀粉饲料。
The present study was conducted to investigate the effects of bile acids (BAs) on the growth, liver function and immunity of the largemouth bass fed high-starch diet. The experiment set three isonitrogenous and isoenergetic semi-purified diets, LS: low-starch diet (5%), HS: high-starch diet (19%) and SB: high-starch diet with BAs (350 mg/kg diet). An 8-week feeding trial was conducted in largemouth bass of initial weight 23.69 +/- 0.13 g. The results indicated that the weight gain (WG) and protein efficiency ratio (PER) of fish fed LS and SB were significantly higher than HS treatment. The superoxide dismutase (SOD) and catalase (CAT) activities of SB group were significantly increased, while malondialdehyde (MDA) content significantly reduced in liver compared with HS group. The activities of alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glucose contents in plasma of SB group were significantly lower than HS treatment, whereas the content of triglyceride (TG) and total cholesterol (TC) in plasma were significantly higher than HS treatment. Additionally, the plasma immunoglobulin count, lysozyme activity and the blood leukocyte count (WBC) in SB group were significantly higher than HS group. The results of paraffin section of liver showed the histopathological alterations were significantly reduced in the SB group compared to HS group. All in all, this study revealed that bile acids supplement could significantly improve growth performance, enhance liver function and immune ability, and alleviate stress responses of M. salmoides fed high-starch diet.