Use of orchiectomy and testosterone replacement to explore meal number-to-meal size relationship in male rats
Use of orchiectomy and testosterone replacement to explore meal number-to-meal size relationship in male rats
复制标题
DOI：
10.1152/ajpregu.1999.276.5.r1366
复制
发表时间：
1999-05-01
影响因子：
2.8
通讯作者：
Varma, M
中科院分区：
文献类型：
--
作者：
Chai, JK;Blaha, V;Varma, M
由于食物摄入量是进食数量和进食大小的函数，而且性别相关的激素参与了进食调节，我们探索了睾丸切除和睾酮替代对进食数量和进食大小关系的影响以及由此导致的进食模式的变化-在成年雄性大鼠中，随机分为睾丸切除组和假手术组。用食鼠仪测量去势前1wk、去势后2wk和睾酮替代后8d的摄食指数。切除会立即改变进食量与大小的关系，导致1)摄食模式的改变；2)暗阶段进食量的显著减少；3)暗阶段进食量的显著增加，但不足以抵消暗阶段进食量的减少，因此暗阶段进食量显著减少；4)光期进食量没有显著变化；以及5)进食量增加导致光期进食量增加，这抵消了暗周期进食量的减少，导致切除后进食量没有显著变化。睾丸激素替代显著逆转了睾丸切除对进食数量与进食大小关系的影响，恢复了进食模式。数据表明，雄激素会立即影响进餐数量与进餐大小的关系。睾丸切除后的发病速度表明，睾酮对食物摄入量的影响也可能部分通过非基因组效应发生。
Because food intake is a function of meal number and meal size and because gender-related hormones are involved in feeding regulation, we explored effects of orchiectomy and testosterone replacement on the relationship between meal number and size and changes-in resulting feeding patterns in adult male rats, randomized into orchiectomy and sham-operation groups. A rat eater meter measured feeding indexes for 1 wk before and 2 wk after castration and during 8 days of testosterone replacement. Orchiectomy leads to an immediate change in the meal number-to-size relationship, resulting in 1) change in pattern of feeding; 2) a significant; decrease in dark-phase meal number; 3) a significant increase in dark-phase meal size, but insufficient to offset decrease in meal number, so total food intake significantly decreased during dark phase; 4) no significant change in light-phase meal number; and 5) an increase in meal size leading to an increased food intake during light phase, which offset decreased food intake in dark cycle and resulted in no net significant change in food intake after orchiectomy. Testosterone replacement acutely reversed effects of orchiectomy on meal number-to-meal size relationship, restoring feeding pattern. Data suggest that androgens immediately influence the meal number-to-meal size relationship. The speed of onset seen after orchiectomy suggests that the influence of testosterone on food intake may also occur partially via a nongenomic effect.