Aerobic biodegradation characteristics and metabolic products of quinoline by a Pseudomonas strain.
Aerobic biodegradation characteristics and metabolic products of quinoline by a Pseudomonas strain.
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DOI：
10.1016/j.biortech.2009.05.044
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发表时间：
2009-11
影响因子：
11.4
通讯作者：
Qinghua Sun;Yaohui Bai;Cui Zhao;Ya-na Xiao;Donghui Wen;Xiao-yan Tang
中科院分区：
文献类型：
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作者：
Qinghua Sun;Yaohui Bai;Cui Zhao;Ya-na Xiao;Donghui Wen;Xiao-yan Tang
分离到一株以喹啉为唯一碳源、氮源和能源的细菌BW003，经鉴定为假单胞菌属（Pseudomonasp.BW003），其喹啉降解率为96%~98%，降解时间为3-8h，降解量为192-911mg/l。降解的最佳条件为30 ° C和pH 8。在生物降解过程中，至少有43%的喹啉转化为2-羟基喹啉，0.69%的2-羟基喹啉转化为2，8-二羟基喹啉，进而转化为8-羟基香豆素。同时，喹啉中至少有48%的氮直接转化为氨氮。外加碳源可促进氨氮转化为氮素。进一步的实验表明，除了细胞合成外，BW003通过异养硝化作用将不到6%的氨氮转化为硝酸盐。另外，BW003含有一个大质粒，可能参与喹啉代谢。研究表明，以BW 003为生物强化菌剂，通过控制C/N比，可以去除废水中的喹啉及其代谢产物。
A bacterial strain, BW003, which utilized quinoline as its sole C, N and energy source, was isolated and identified as Pseudomonas sp. BW003 degraded 192–911mg/l quinoline within 3–8h with removal rates ranging from 96% to 98%. The optimum conditions for the degradation were 30°C and pH 8. In the process of biodegradation, at least 43% of quinoline was transformed into 2-hydroxyquinoline, then 0.69% of 2-hydroxyquinoline was transformed into 2,8-dihydroxyquinoline, and then, presumably, into 8-hydroxycoumarin. Meanwhile, at least 48% of the nitrogen in quinoline was directly transformed into ammonia-N. An extra carbon source enhanced the nitrogen transformation from ammonia-N. Further experiments showed that, besides cell synthesis, BW003 transformed less than 6% of ammonia-N into nitrate through heterotrophic nitrification. In addition, BW003 contained a large plasmid, which may be involved in quinoline metabolism. The study indicates that quinoline and its metabolic products can be eliminated from wastewater by controlling the C/N ratio using BW003 as the bioaugmentation inoculum.