Expression and citrullination of keratin in synovial tissue of rheumatoid arthritis
Expression and citrullination of keratin in synovial tissue of rheumatoid arthritis
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DOI：
10.1007/s00296-009-0863-1
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发表时间：
2009-09-01
影响因子：
4
通讯作者：
Yan, Xinfeng
中科院分区：
文献类型：
--
作者：
Chang, Xiaotian;Jian, Xiangdong;Yan, Xinfeng
角蛋白是细胞中间丝的主要成分，其翻译后修饰在细胞分化、凋亡以及疾病状态中起着重要作用。肽基精氨酸转化为瓜氨酸，称为瓜氨酸，特别涉及类风湿性关节炎（RA）的发病机制。免疫组化使用的抗体混合物，广泛地认识到各种角蛋白的形式检测到细胞角蛋白在许多细胞中的区域衬里的滑膜类风湿关节炎。此外，双重免疫荧光标记显示，当表达细胞角蛋白的细胞位于细胞外沉积物附近或接近滑膜外部时，瓜氨酸也呈阳性。Western blot分析表明，瓜氨酸角蛋白纯化RA滑膜组织中的免疫沉淀。以上结果表明RA滑膜中存在瓜氨酸化细胞角蛋白。
Keratin is the main component of cellular intermediate filaments, and its post-translational modification plays an important role in cell differentiation and apoptosis, as well as disease states. The conversion of peptidylarginine to citrulline, termed citrullination, is particularly involved in the pathogenesis of rheumatoid arthritis (RA). Immunohistochemistry using an antibody mixture that broadly recognized various keratin forms detected cytokeratin in many cells in the area lining the synovial membrane of RA. Furthermore, double immuno-florescent labeling showed that the cells expressing cytokeratin were also positive for citrulline when they were in the vicinity of extracellular deposits or approached the exterior of the synovial membrane. Western blot analysis demonstrated citrullination of keratin purified from RA synovial tissue by immuno-precipitation. The above results indicate the presence of citrullinated cytokeratin in synovial membranes in RA.