Production, Purification, and Antibiofilm Activity of a Novel Exopolysaccharide from Arthrobacter sp. B4
Production, Purification, and Antibiofilm Activity of a Novel Exopolysaccharide from Arthrobacter sp. B4
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节杆菌属新型胞外多糖的生产、纯化和抗菌膜活性。
DOI：
10.1080/10826068.2014.907180
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发表时间：
2015-01-01
影响因子：
2.9
通讯作者：
Zhao, Zhiwen
中科院分区：
文献类型：
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作者：
Li, Yumei;Li, Qiang;Zhao, Zhiwen
节杆菌产生一种新型胞外多糖 (EPS)，即 B4-EPS。 B4。采用响应面法 (RSM) 来优化发酵培养基，以提高 B4-EPS 产量。基于 Plackett-Burman 设计 (PBD)，选择葡萄糖、酵母提取物和 KH2PO4 作为显着变量，并通过中心复合设计 (CCD) 进一步优化。根据响应面和典型分析，最佳培养基组成为葡萄糖16.94 g/L、酵母抽提物2.33 g/L、KH2PO4 5.32 g/L。在此条件下，分批发酵72小时后B4-EPS的最大产量达到约8.54 g/L，与预测值（8.52 g/L）非常接近。此外，B4-EPS通过柱色谱法进行精制。收集主要均质级分（B4-EPS1）并用于抗生物膜活性测定。 B4-EPS1 对铜绿假单胞菌 PAO1 生物膜形成具有剂量依赖性抑制作用，但没有抗菌活性。在50μg/mL B4-EPS1存在下，约86.1%的铜绿假单胞菌PAO1生物膜形成被抑制，这比同行公布的数据更有效。此外，B4-EPS1 可以防止其他菌株生物膜的形成。这些数据表明 B4-EPS 可能是未来对抗细菌生物膜的一种有前景的策略。
A novel exopolysaccharide (EPS), namely, B4-EPS, is produced by Arthrobacter sp. B4. Response surface methodology (RSM) was employed to optimize the fermentation medium for increasing B4-EPS production. Based on Plackett-Burman design (PBD), glucose, yeast extract, and KH2PO4 were selected as significant variables, which were further optimized by a central composite design (CCD). According to response surface and canonical analysis, the optimal medium was composed of 16.94 g/L glucose, 2.33g/L yeast extract, and 5.32 g/L KH2PO4. Under this condition, the maximum yield of B4-EPS reached about 8.54 g/L after 72 hr of batch fermentation, which was pretty close to the predicted value (8.52 g/L). Furthermore, B4-EPS was refined by column chromatography. The main homogeneous fraction (B4-EPS1) was collected and applied to assay of antibiofilm activity. B4-EPS1 exhibited a dose-dependent inhibitory effect on biofilm formation of Pseudomonas aeruginosa PAO1 without antibacterial activity. About 86.1% of biofilm formation of P. aeruginosa PAO1 was inhibited in the presence of 50 mu g/mL B4-EPS1, which was more effective than the peer published data. Moreover, B4-EPS1 could prevent biofilm formation of other strains. These data suggest B4-EPS may represent a promising strategy to combat bacterial biofilms in the future.