Dynamics of Presynaptic Diacylglycerol in a Sensory Neuron Encode Differences between Past and Current Stimulus Intensity
Dynamics of Presynaptic Diacylglycerol in a Sensory Neuron Encode Differences between Past and Current Stimulus Intensity
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DOI：
10.1016/j.celrep.2017.08.038
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发表时间：
2017-09-05
期刊：
影响因子：
8.8
通讯作者：
Iino, Yuichi
中科院分区：
文献类型：
--
作者：
Ohno, Hayao;Sakai, Naoko;Iino, Yuichi
增强感官刺激的强度使动物能够成功地应对不断变化的环境条件，并有助于认知功能，如听觉和视觉工作记忆。然而，神经系统如何处理过去和当前的刺激强度在分子水平上基本上是未知的。在这里，我们采用在体内的二酰基甘油（DAG）成像的ASER味觉神经元的秀丽隐杆线虫，并表明，周围的盐浓度和食物之间的关联学习可以解释突触前DAG的变化。DAG的丰度通过ASER中的感觉转导响应于外部盐浓度变化而调节，并且可以编码过去和当前盐浓度之间的差异。DAG的动力学调节下游的突触胰岛素/磷脂酰肌醇3-激酶（PI 3 K）/Akt途径，调节饥饿诱导的行为可塑性。这些结果提供了对单个神经元如何存储过去的输入强度并产生适当的行为反应的见解。
Memorizing the intensity of sensory stimuli enables animals to successfully deal with changing environmental conditions and contributes to cognitive functions such as auditory and visual working memory. However, how nervous systems process past and current stimulus intensity is largely unknown at the molecular level. Here, we employ in vivo diacylglycerol (DAG) imaging in the ASER taste neuron of Caenorhabditis elegans and demonstrate that associative learning between ambient salt concentrations and food can be explained by changes in presynaptic DAG. The abundance of DAG is regulated in response to external salt concentration changes via sensory transduction in ASER and can encode differences between past and current salt concentrations. The DAG dynamics are modulated downstream of the synaptic insulin/phosphatidylinositol 3-kinase (PI3K)/Akt pathway, which regulates the behavioral plasticity induced by starvation. These results provide insights into how a single neuron stores past input intensity and generates appropriate behavioral responses.