EEG evaluation of reflex testing as assessment of depth of pentobarbital anaesthesia in the rat
EEG evaluation of reflex testing as assessment of depth of pentobarbital anaesthesia in the rat
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DOI：
10.1258/002367799780578570
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发表时间：
1999-01-01
影响因子：
2.4
通讯作者：
Hellebrekers, LJ
中科院分区：
文献类型：
--
作者：
Haberham, ZL;van den Brom, WE;Hellebrekers, LJ
应用脑电图 (EEG) 来评估用单次腹腔内剂量戊巴比妥麻醉的大鼠中爪捏反射作为麻醉深度指标的有效性。麻醉诱导后，以动物保持直立姿势的能力迅速丧失为特征，11 只大鼠中有 10 只的脑电图以阵发性（爆发抑制）活动为主，与意识不清有关。 11 只大鼠中有 7 只在出现阵发性脑电图活动后，爪捏反射消失。然而，11 只动物中的 4 只仍存在捏爪反射，这表明该反应与皮质活动无关。七只大鼠中的 5 只在爪捏反射恢复时，脑电图仍显示阵发性活动。然而，在另外两只大鼠中，脑电图分别在反射恢复前 4 分钟和 21 分钟恢复到与清醒动物相似的模式。这些数据表明：在戊巴比妥麻醉的大鼠中，爪捏反射的存在与大脑皮层电活动的抑制水平无关，因此可能与意识水平无关。基于这些发现，得出的结论是，爪子捏反射作为麻醉深度的唯一指标是不可靠的。
Electroencephalography (EEG) was applied to evaluate the validity of the paw pinch reflex as an indicator of anaesthetic depth in rats which are anaesthetized with a single intraperitoneal dose of pentobarbital.After induction of the anaesthesia, characterized by the rapid loss of the animals' ability to maintain upright posture, the EEG of 10 out of 11 rats was dominated by paroxysmal (burst suppression) activity, associated with unconsciousness. In seven out of 11 rats, the paw pinch reflex was lost after onset of paroxysmal electroencephalographic activity. However, the paw pinch reflex remained present in four out of 11 animals, demonstrating that the response is independent of cortical activity.In five out of seven rats, the EEG still showed paroxysmal activity when the paw pinch reflex was regained. However, in two other rats the EEG returned to a pattern similar to that shown by awake animals, 4 and 21 min respectively, before the reflex was regained.These data indicate: that in the pentobarbital-anaesthetized rat, presence of the paw pinch reflex is not related to the level of depression of electrical activity in the cerebral cortex, and consequently is probably not related to the level of consciousness. Based upon these findings it is concluded that the paw pinch reflex is unreliable as a sole indicator of anaesthetic depth.