PHYSICAL PROPERTIES OF CHICK INTERFERON
PHYSICAL PROPERTIES OF CHICK INTERFERON
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DOI：
10.1128/jb.89.2.462-469.1965
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发表时间：
1965-01-01
影响因子：
3.2
通讯作者：
LEVY, AH
中科院分区：
文献类型：
--
作者：
KREUZ, LE;LEVY, AH
在氯化物密度梯度和sephadex g-100柱上，通过区域离心和平衡沉积物确定鸡 - 丝晶干扰素的沉积系数，扩散系数和分子量。纯化的干扰素的数量不足以允许通过化学或物理手段进行直接分析；因此，通过生物测定确定其相对浓度。在所有实验中，I125人类血清白蛋白均用作内部参考。雏鸡干扰素的沉积系数为2.2至2.3s；扩散系数为9.5 x 10-7 cm2 sec-1。根据沉积和扩散系数计算分子量为26,000，并从平衡 - 补充分析中获得了25,000至34,000 dalton的范围。
The sedimentation coefficient, diffusion coefficient, and molecular weight of chick-embryo interferon were determined by zone centrifugation and equilibrium sedimentation in caseium chloride density gradients, and by chromato-graphy on Sephadex G-100 columns. Purified interferon is not available in quantities sufficient to permit direct analysis by chemical or physical means; its relative concentrations were determined, therefore, by bioassay. I125-human serum albumin was used as an internal reference in all experiments. The sedimentation coefficient of chick-embryo interferon is 2.2 to 2.3S; the diffusion coefficient is 9.5 X 10-7 cm2 sec-1. A molecular weight of 26,000 was calculated from the sedimentation and diffusion coefficients, and a range of 25,000 to 34,000 daltons was obtained from equilibrium-sedimentatmon analyses.