Coexpression of the cannabinoid receptor type 1 with dopamine and serotonin receptors in distinct neuronal subpopulations of the adult mouse forebrain
Coexpression of the cannabinoid receptor type 1 with dopamine and serotonin receptors in distinct neuronal subpopulations of the adult mouse forebrain
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DOI：
10.1016/s0306-4522(01)00509-7
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发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
Lutz, B
中科院分区：
文献类型：
--
作者：
Hermann, H;Marsicano, G;Lutz, B
大麻素受体1型（CB 1）显示出不寻常的特性，包括抑制或刺激腺苷酸环化酶的双重能力，以及比大多数G蛋白偶联受体高得多的脑密度。与多巴胺和5-羟色胺受体的重叠表达模式一起，这表明CB 1调节多巴胺和5-羟色胺系统功能的潜力。事实上，药理学研究为CB 1和这些神经递质系统的受体之间的交叉对话提供了证据。在试图获得进一步的见解CB 1和多巴胺受体和5-羟色胺受体之间可能的功能和/或结构的相互作用，我们进行了双标记原位杂交在细胞水平上的小鼠前脑部分结合地高辛标记的核糖体探针CB 1与S-35标记的核糖体探针多巴胺受体D1和D2。和5-羟色胺受体5-HT 1B和5-HT 3。作为一般规则，我们发现，CB 1与D1，D2和5-HT 1B共定位仅在低表达CB 1的细胞中，这些细胞是主要的投射神经元，而CB 1与5-HT 3共表达也在高表达CB 1的细胞中观察到，这些细胞被认为主要是GABA能的。在纹状体和嗅结节中，CB 1与D1、D2和5-HT 1B高度共表达。在整个海马结构中，CB 1与D2、5-HT 1B和5-HT 3共表达。在大脑皮层。仅用5-HT 1B和5-HT 3检测到共表达。总之，大麻素系统与多巴胺和5-羟色胺受体共定位的独特模式正在出现，因此。它与这些神经递质系统的相互作用可能有不同的机制。(C)2002年IBRO。由Elsevier Science Ltd.出版，版权所有。
The cannabinoid receptor type 1 (CB1) displays unusual properties, including the dual capacity to inhibit or stimulate adenylate cyclase and a brain density considerably higher than the majority of G protein-coupled receptors. Together with overlapping expression patterns of dopamine and serotonin receptors this suggests a potential of CB1 to modulate the function of the dopamine and serotonin system. Indeed, pharmacological studies provide evidence for cross-talks between CB1 and receptors of these neurotransmitter systems. In trying to obtain further insights into possible functional and/or structural interactions between CB1 and the dopamine receptors and the serotonin receptors, we performed double-label in situ hybridization at the cellular level on mouse forebrain sections by combining a digoxigenin-labelled riboprobe for CB1 with S-35-labelled riboprobes for dopamine receptors D1 and D2. and for serotonin receptors 5-HT1B and 5-HT3, respectively. As a general rule, we found that CB1 colocalizes with D1, D2 and 5-HT1B only in low-CB1-expressing cells which are principal projecting neurons, whereas CB1 coexpression with 5-HT3 was also observed in high-CB1-expressing cells which are considered to be mostly GABAergic. In striatum and olfactory tubercle, CB1 is coexpressed to a high extent with D1, D2 and 5-HT1B. Throughout the hippocampal formation, CB1 is coexpressed with D2, 5-HT1B and 5-HT3. In the neocortex. coexpression was detected only with 5-HT1B and 5-HT3. In summary a distinct pattern is emerging for the cannabinoid system with regard to its colocalization with dopamine and serotonin receptors and, therefore. it is likely that different mechanisms underlie its cross-talk with these neurotransmitter systems. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.