RhoE enhances multidrug resistance of gastric cancer cells by suppressing Bax
RhoE enhances multidrug resistance of gastric cancer cells by suppressing Bax
复制标题
RhoE 通过抑制 Bax 增强胃癌细胞的多药耐药性。
DOI：
10.1016/j.bbrc.2008.12.044
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发表时间：
2009-02-06
影响因子：
3.1
通讯作者：
Fan, Daiming
中科院分区：
文献类型：
--
作者：
Li, Kai;Lu, Yuanyuan;Fan, Daiming
我们之前曾报道过 RhoE 在 SGC7901/VCR 细胞系中过表达。然而，RhoE 在胃癌多药耐药性发展中的潜在作用尚不清楚。在本研究中，RhoE增强了SGC7901细胞对多种抗肿瘤药物的耐药性。 RhoE过表达不改变SGC7901细胞内阿霉素的积累，也不改变P-gp和MRP-1的表达，但保护SGC7901细胞免于长春新碱诱导的细胞凋亡。研究发现 RhoE 在转录后水平下调 Bax 的表达。蛋白质印迹显示 RhoE 对 Caspase 家族蛋白的活性没有影响。简而言之，我们的研究表明，RhoE可能通过降低转录后水平Bax的表达来促进胃癌细胞的多药耐药表型，从而抑制长春新碱诱导的细胞凋亡。 (C) 2008 Elsevier Inc. 保留所有权利。
We have previously reported that RhoE is overexpressed in the SGC7901/VCR cell line. However, the potential role of RhoE in the development of multidrug resistance of gastric cancer is unknown. In the present study, RhoE enhanced the resistance of SGC7901 cells to several kinds of antitumor drugs. RhoE overexpression did not alter the intracellular adriamycin accumulation of SGC7901 cells nor the expression of P-gp and MRP-1, but protected SGC7901 cells from vincristine-induced apoptosis. RhoE was found to downregulate the expression of Bax at a posttranscriptional level. Western blot revealed no effects of RhoE on the activities of the Caspase family of proteins. in brief, our study demonstrated that RhoE may promote the multidrug resistance phenotype of gastric cancer cells by decreasing the expression of Bax at posttranscriptional level, thus inhibiting vincristine-induced apoptosis. (C) 2008 Elsevier Inc. All rights reserved.