Glia maturation factor gamma (GMFG): a cytokine-responsive protein during hematopoietic lineage development and its functional genomics analysis.
Glia maturation factor gamma (GMFG): a cytokine-responsive protein during hematopoietic lineage development and its functional genomics analysis.
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DOI：
10.1016/s1672-0229(06)60027-2
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发表时间：
2006-08
期刊：
影响因子：
--
通讯作者：
Rodgers GP
中科院分区：
文献类型：
--
作者：
Shi Y;Chen L;Liotta LA;Wan HH;Rodgers GP
使用受控干细胞分化、基于二维凝胶电泳的蛋白质组学和功能基因组学技术评估人类造血功能。我们首次报道神经胶质成熟因子γ（GMFG）是促红细胞生成素诱导和粒细胞集落刺激因子诱导的造血谱系发育中的细胞因子反应蛋白。全球功能基因组学分析的结果表明，GMFG 还具有其他几个特征：造血组织特异性基因表达、富含高分造血特异性转录因子的启动子，以及可能与基本血液/免疫系统的分子共进化。根据我们的研究结果，我们假设 GMFG 是一种造血特异性蛋白，可能介导人类造血干细胞的多能性和谱系定向。
Human hematopoiesis was evaluated using the techniques of controlled stem cell differentiation, two-dimensional gel electrophoresis-based proteomics, and functional genomics. We provide the first report that glia maturation factor gamma (GMFG) is a cytokine-responsive protein in erythropoietin-induced and granulocyte-colony stimulating factor-induced hematopoietic lineage development. Results from global functional genomics analysis indicate that GMFG possesses several other features: hematopoietic tissue-specific gene expression, a promoter concentrated with high-score hematopoiesis-specific transcription factors, and possible molecular coevolution with a rudimentary blood/immune system. On the basis of our findings, we hypothesize that GMFG is a hematopoietic-specific protein that may mediate the pluripotentiality and lineage commitment of human hematopoietic stem cells.