Essential hypertension: A filtered serum based metabolomics study.
Essential hypertension: A filtered serum based metabolomics study.
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DOI：
10.1038/s41598-017-02289-9
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发表时间：
2017-05-19
影响因子：
4.6
通讯作者：
Mahdi AA
中科院分区：
文献类型：
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作者：
Ameta K;Gupta A;Kumar S;Sethi R;Kumar D;Mahdi AA
尽管血压测量方法简单可靠，但由于症状出现的延迟，高血压的筛查往往被忽视。对高血压的生化变化的探讨将是一项受欢迎的资产，有助于深入了解高血压的机制。用800 MHz的核磁共振波谱分析了高血压患者和59名健康对照的过滤后血清样品。根据核磁共振数据的受试者工作特征(ROC)曲线，应用主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)，揭示了显著扰动的代谢物：丙氨酸、精氨酸、蛋氨酸、丙酮酸、腺嘌呤和尿嘧啶。这组代谢物正确地将99%的病例与HC区分开来，并且在单纯性舒张压(DBP)升高和收缩-舒张压联合升高的病例中也显示出良好的相关性。EH的质子核磁共振代谢组学可能有助于确定相关的生物标志物，并可作为临床评估的替代诊断工具。
Despite the easy and reliable methods of blood pressure measurement, the screening of essential hypertension (EH) is usually ignored due to delayed onset of symptoms. A probe into the biochemical changes in hypertension would serve as a welcome asset to provide insight into the mechanistic aspects of EH. Filtered serum samples from 64 EH patients and 59 healthy controls (HC) were analysed using 800 MHz nuclear magnetic resonance (NMR) spectroscopy. Application of principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) following receiver operating characteristic (ROC) curve of NMR data reveals significantly perturbed metabolites: alanine, arginine, methionine, pyruvate, adenine, and uracil. This set of metabolites correctly classified 99% of cases from HC and also showed excellent correlation in both isolated elevated diastolic blood pressure (DBP) cases and combined elevated systolic-diastolic blood pressure cases. Proton NMR metabolomics of EH may prove helpful in defining associated biomarkers and serve as an alternate diagnostic tool with judicious clinical assessment.