Sesquiterpene, alkene, and alkane hydrocarbons in virgin olive oils of different varieties and geographical origins
Sesquiterpene, alkene, and alkane hydrocarbons in virgin olive oils of different varieties and geographical origins
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DOI：
10.1021/jf001271w
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发表时间：
2001-07-01
影响因子：
6.1
通讯作者：
Conte, LS
中科院分区：
文献类型：
--
作者：
Bortolomeazzi, R;Berno, P;Conte, LS
分析了30种初榨橄榄油的烃馏分，特别关注倍半萜烯。油样品来自不同的地理来源，并从不同的橄榄品种中获得。烃馏分通过油的不皂化馏分的硅胶柱色谱法分离。然后，根据倍半萜烃的不饱和度，通过AgNO 3 TLC和硅胶AgNO 3柱色谱法对倍半萜烃进行分馏。倍半萜的组成比以前报道的更复杂。在检测到的31种倍半萜中，通过两种不同极性毛细管柱的线性保留指数和质谱与文献报道的结果进行比较，初步鉴定了24种倍半萜。所分析的油中倍半萜烯的总浓度范围为约2至37 ppm。在倍半萜中，含量较高的是α-法呢烯、α-可巴烯、艾瑞莫菲烯和α-木乌罗烯.通过TLC AgNO 3分离烃馏分中存在的烯烃，并通过其二甲基二硫醚衍生物的GC-MS进行表征。从C-22到C-27，8-十七碳烯和6，10-二甲基-1-十一碳烯的一系列n-δ（9）-烯烃被检测到。在正构烷烃中，具有奇数碳原子的那些在所有分析的油中占主导地位，最常见的是C-23、C-25、C-27和C-29。正烯烃的浓度范围为约0.5至2 ppm，而正烷烃的浓度范围为30至177 ppm。
The hydrocarbon fraction of 30 virgin olive oils was analyzed, focusing in particular on the sesquiterpenes. The oil samples were of different geographical origins and obtained from different olive varieties. The hydrocarbon fraction was isolated by silica gel column chromatography of the unsaponifiable fraction of the oils. The sesquiterpene hydrocarbons were then fractionated, on the basis of their degree of unsaturation, by AgNO3 TLC and silica gel AgNO3 column chromatography. The composition of the sesquiterpenes was more complex than previously reported. Among the 31 sesquiterpenes detected, 24 have been tentatively identified, by comparison of the linear retention indices on two capillary columns of different polarities and mass spectra with those reported in the literature. The total concentration of the sesquiterpenes in the oils analyzed ranged from about 2 to 37 ppm. Among the sesquiterpenes the more abundant were alpha -farnesene, alpha -copaene, eremophyllene, and alpha -muurolene. The alkenes present in the hydrocarbon fraction were isolated by TLC AgNO3 and characterized by GC-MS of their dimethyl disulfide derivatives. The series of n-Delta (9)-alkenes from C-22 to C-27, 8-heptadecene, and 6,10-dimethyl-1-undecene were detected. Among the n-alkanes, those with an odd number of carbon atoms predominated in all of the analyzed oils, the most common being C-23, C-25, C-27, and C-29. The concentration of the n-alkenes ranged from about 0.5 to 2 ppm, whereas for the n-alkanes the range was from 30 to 177 ppm.