Antibacterial Properties of Triethoxysilylpropyl Succinic Anhydride Silane (TESPSA) on Titanium Dental Implants
Antibacterial Properties of Triethoxysilylpropyl Succinic Anhydride Silane (TESPSA) on Titanium Dental Implants
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DOI：
10.3390/polym12040773
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发表时间：
2020-04-01
期刊：
影响因子：
5
通讯作者：
Gil, Francisco Javier
中科院分区：
文献类型：
--
作者：
Buxadera-Palomero, Judit;Godoy-Gallardo, Maria;Gil, Francisco Javier
与牙种植体相关的感染是一种常见的并发症，最终可能导致种植体失败，从而带来重大的健康和经济成本。为了抵御这些感染，本文探讨了三乙氧基甲硅烷基丙基琥珀酸酐（TESPSA，TSP）硅烷固定到牙种植体上，两个不同的单种生物膜和口腔菌斑与涂层钛样品的相互作用。为此，来自先前机加工的钛盘首先通过NaOH处理活化，并进一步用TESPSA硅烷官能化。通过扫描电子显微镜和X射线光电子能谱分析观察到多孔钛酸钠表面，证实了钛样品上TESPSA的存在（Ti-N-TSP为8.4%）。此外，乳酸脱氢酶测定得出结论，TESPSA对人成纤维细胞的活力没有负面影响。重要的是，使用活细菌粘附测定法研究了改性表面对血链球菌、唾液乳杆菌和口腔菌斑的体外作用。在所有情况下都实现了显著减少，但正如预期的那样，对简单单物种生物膜（死/活比率为0.4）和完全口腔生物膜（死/活比率为0.6）具有不同的有效性。然而，这种方法具有提供具有抗微生物特性的牙科植入物的巨大潜力。
Infections related to dental implants are a common complication that can ultimately lead to implant failure, and thereby carries significant health and economic costs. In order to ward off these infections, this paper explores the immobilization of triethoxysilylpropyl succinic anhydride (TESPSA, TSP) silane onto dental implants, and the interaction of two distinct monospecies biofilms and an oral plaque with the coated titanium samples. To this end, titanium disks from prior machining were first activated by a NaOH treatment and further functionalized with TESPSA silane. A porous sodium titanate surface was observed by scanning electron microscopy and X-ray photoelectron spectroscopy analyses confirmed the presence of TESPSA on the titanium samples (8.4% for Ti-N-TSP). Furthermore, a lactate dehydrogenase assay concluded that TESPSA did not have a negative effect on the viability of human fibroblasts. Importantly, the in vitro effect of modified surfaces against Streptococcus sanguinis, Lactobacillus salivarius and oral plaque were studied using a viable bacterial adhesion assay. A significant reduction was achieved in all cases but, as expected, with different effectiveness against simple mono-species biofilm (ratio dead/live of 0.4) and complete oral biofilm (ratio dead/live of 0.6). Nevertheless, this approach holds a great potential to provide dental implants with antimicrobial properties.