The herpesvirus saimiri ORF73 gene product interacts with host-cell mitotic chromosomes and self-associates via its C terminus
The herpesvirus saimiri ORF73 gene product interacts with host-cell mitotic chromosomes and self-associates via its C terminus
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DOI：
10.1099/vir.0.19437-0
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发表时间：
2004-01-01
影响因子：
3.8
通讯作者：
Whitehouse, A
中科院分区：
文献类型：
--
作者：
Calderwood, MA;Hall, KT;Whitehouse, A
疱疹病毒;猴（HVS） ORF73基因产物与卡波西肉瘤相关疱疹病毒（KSHV）的ORF73蛋白具有有限的同源性。ORF73在体外HVS潜伏期模型中表达，其中基因组持续存在为非整合的圆形片段。这表明它可能与KSHV ORF73一样，通过将病毒基因组拴在宿主细胞染色体上，来维持个体的生存。本文描述了ORF73与宿主有丝分裂染色体的关联。缺失分析表明远端123 aa。ORF73蛋白的一部分是有丝分裂染色体定位和自我结合所必需的。此外，极端C端的缺失破坏了自结合和宿主有丝分裂染色体共定位。这表明HVS ORF73在个体维持中具有与KSHV ORF73相似的作用，并且ORF73与宿主有丝分裂染色体的关联依赖于其形成多聚体的能力。
The herpesvirus; saimiri (HVS) ORF73 gene product shares limited homology with the ORF73 protein of Kaposi's sarcoma-associated herpesvirus (KSHV). ORF73 is expressed in an in vitro model of HVS latency, where the genome persists as a non-integrated circular episome. This suggests it may have a similar role to KSHV ORF73 in episomal maintenance, by tethering viral genomes to host-cell chromosomes. Here, the association of ORF73 with host mitotic chromosomes is described. Deletion analysis demonstrates that the distal 123 aa. of the ORF73 protein are required for mitotic chromosomal localization and for self-association. Moreover, deletion of the extreme C terminus disrupts both self-association and host mitotic chromosome colocalization. This suggests that HVS ORF73 has a similar role to KSHV ORF73 in episomal maintenance and that association of ORF73 to host mitotic chromosomes is dependent on its ability to form multimers.