Retinoblastoma-binding protein 2 (Rbp2) potentiates nuclear hormone receptor-mediated transcription
Retinoblastoma-binding protein 2 (Rbp2) potentiates nuclear hormone receptor-mediated transcription
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DOI：
10.1074/jbc.m100313200
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发表时间：
2001-07-27
影响因子：
4.8
通讯作者：
Hong, WJ
中科院分区：
文献类型：
--
作者：
Chan, SW;Hong, WJ
视网膜母细胞瘤结合蛋白2（Retinoblastoma-binding protein 2，Rbp 2）最初被鉴定为视网膜母细胞瘤蛋白（retinoblastoma protein，RB）口袋结构域结合蛋白。尽管Rbp 2已被证明与RB、p107、TATA结合蛋白和T细胞癌基因菱蛋白-2相互作用，但Rbp 2的生理功能仍不清楚。在这里，我们证明，Rbp 2不仅结合到核受体（NR），但也增强了他们介导的转录。Rbp 2与NR的DNA结合结构域相互作用，并以AF-2依赖的方式增强NR介导的转录。Rbp 2的N-末端和C-末端结构域对于Rbp 2对NR的反式激活活性都是至关重要的。C末端是NR相互作用区。此外，RB的功能是使Rbp 2对NR转录的影响最大化。这些结果表明，Rbp 2是NR的共调节子，并定义了Rbp 2在NR介导的转录中的潜在作用。
Retinoblastoma-binding protein 2 (Rbp2) was originally identified as a retinoblastoma protein (RB) pocket domain-binding protein. Although Rbp2 has been shown to interact with RB, p107, TATA-binding protein, and T-cell oncogene rhombotin-2, the physiological function of Rbp2 remains unclear. Here we demonstrate that Rbp2 not only binds to nuclear receptors (NRs) but also enhances the transcription mediated by them. Rbp2 interacts with the DNA-binding domains of NRs and potentiates NR-mediated transcription in an AF-2-dependent manner. Both the N-terminal and C-terminal domains of Rbp2 are critical for the transactivation activity of Rbp2 on NRs. The C terminus is the NR-interacting region. In addition, RB functions in maximizing the effect of Rbp2 on the transcription by NRs. These results suggest that Rbp2 is a coregulator of NRs and define a potential role for Rbp2 in NR-mediated transcription.