Response of the hyperthermophilic archaeon Sulfolobus solfataricus to UV damage
Response of the hyperthermophilic archaeon Sulfolobus solfataricus to UV damage
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DOI：
10.1128/jb.01016-07
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发表时间：
2007-12-01
影响因子：
3.2
通讯作者：
Schleper, Christa
中科院分区：
文献类型：
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作者：
Frols, Sabrina;Gordon, Paul M. K.;Schleper, Christa
为了研究高温好氧细菌Sulfolobus solfararicus对紫外线损伤的全基因组转录反应，我们使用了高密度DNA芯片，覆盖了宿主基因组上编码的3368个遗传特征，以及几个染色体外遗传元件的基因。虽然没有观察到可能参与直接DNA损伤逆转的基因显著上调，但可以解剖涉及55个基因的特异转录紫外线反应。虽然流式细胞术显示细胞周期只有轻微的扰动，但观察到CDC6复制启动子基因的转录水平有很强的调节。编码Mre11和Rad50同源物的操纵子上调指向诱导重组修复。与此相一致的是，紫外线照射后2~8h可观察到DNA双链断裂，这可能是由于DNA损伤部位的复制叉坍塌所致。对可能编码菌毛形成功能的基因的强烈转录诱导表明，接合活性可能导致遗传物质的增强交换。为了支持这一点，统计显微镜分析表明，在紫外线照射下形成了大的细胞聚集体。总之，这为重组修复和接合事件之间的联系提供了支持证据。
In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements. While no significant up-regulation of genes potentially involved in direct DNA damage reversal was observed, a specific transcriptional UV response involving 55 genes could be dissected. Although flow cytometry showed only modest perturbation of the cell cycle, strong modulation of the transcript levels of the Cdc6 replication initiator genes was observed. Up-regulation of an operon encoding Mre11 and Rad50 homologs pointed to induction of recombinational repair. Consistent with this, DNA double-strand breaks were observed between 2 and 8 h after UV treatment, possibly resulting from replication fork collapse at damaged DNA sites. The strong transcriptional induction of genes which potentially encode functions for pilus formation suggested that conjugational activity might lead to enhanced exchange of genetic material. In support of this, a statistical microscopic analysis demonstrated that large cell aggregates formed upon UV exposure. Together, this provided supporting evidence to a link between recombinational repair and conjugation events.