Surface-Enhanced, Spatially Offset Raman Spectroscopy (SESORS) in Tissue Analogues
Surface-Enhanced, Spatially Offset Raman Spectroscopy (SESORS) in Tissue Analogues
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DOI：
10.1021/acsami.7b09197
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发表时间：
2017-08-02
影响因子：
9.5
通讯作者：
Graham, Duncan
中科院分区：
文献类型：
--
作者：
Asiala, Steven M.;Shand, Neil C.;Graham, Duncan
表面增强空间偏移拉曼光谱（SESORS）将表面增强拉曼光谱（Sers）提供的灵敏度的显著增强与空间偏移拉曼光谱的非侵入性地下采样能力相结合。总之，这些技术在体内拉曼测量显示出很大的希望。在这里，我们提出了一个步骤，这种技术，通过组织模拟物的六个已知的和不同的厚度，具有大量的不同的空间偏移，在后向散射光学几何演示SESORS。这是通过在载玻片上旋涂SERS活性纳米颗粒（NP）并分别监测来自NP和组织切片的相对光谱贡献来实现的，作为组织厚度和收集探针的空间偏移的函数。结果表明，SESORS优于单独用于此目的的Sers，NP信号可以在>6.75 mm的组织厚度下获得，并且更大的组织厚度需要更大的空间偏移以最大化NP信号，所有这些都具有针对实用性优化的光学几何结构。该演示代表了SESORS用于非侵入性体内分析的实施向前迈出的一步。
Surface-enhanced, spatially offset Raman spectroscopy (SESORS) combines the remarkable enhancements in sensitivity afforded by surface-enhanced Raman spectroscopy (SERS) with the non-invasive, subsurface sampling capabilities of spatially offset Raman spectroscopy. Taken together, these techniques show great promise for in vivo Raman measurements. Herein, we present a step forward for this technique, demonstrating SESORS through tissue analogues of six known and varied thicknesses, with a large number of distinct spatial offsets, in a backscattering optical geometry. This is accomplished by spin-coating SERS-active nanoparticles (NPs) on glass slides and monitoring the relative spectral contribution from the NPs and tissue sections, respectively, as a function of both the tissue thickness and the spatial offset of the collection probe. The results show that SESORS outperforms SERS alone for this purpose, the NP signal can be attained at tissue thicknesses of >6.75 mm, and greater tissue thicknesses require greater spatial offsets to maximize the NP signal, all with an optical geometry optimized for utility. This demonstration represents a step forward toward the implementation of SESORS for non-invasive, in vivo analysis.